Impacts of long-term nitrogen addition, watering and mowing on ammonia oxidizers, denitrifiers and plant communities in a temperate steppe

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Impacts of long-term nitrogen addition, watering and mowing on ammonia oxidizers, denitrifiers and plant communities in a temperate steppe
	He, Ji-Zheng; Zhang, Cui-Jing; Yang, Zhong-Ling; Shen, Ju-Pei; Sun, Yi-Fei; Wang, Jun-Tao; Han, Hong-Yan; Wan, Shi-Qiang; Zhang, Li-Mei
刊名	APPLIED SOIL ECOLOGY
	2018-09-01
卷号	130 页码:241-250
关键词	Global changes Ammonia oxidizers Denitrifiers Community structure Plant biomass
ISSN号	0929-1393
文献子类	Article
英文摘要	Global changes in nitrogen (N) deposition, precipitation patterns and land use could have an impact on the biogeochemical N cycle mediated by microorganisms. Microbial responses to global changes have been generally evaluated in short-term studies, while the long-term effects are not well understood. In this study, the interactive effects of multiple global change factors on N-cycling microorganisms and their interactions with plants were investigated in a 9-year field experiment with N addition, watering and mowing in a temperate steppe in northern China. Quantitative PCR, terminal restriction fragment length polymorphism (T-RFLP) and clone library were used to analyze the abundance and community structure of ammonia oxidizers and denitrifiers, respectively. Bacterial 16S rRNA gene abundance significantly increased under mowing, but the functional gene abundances including amoA, nirS, nirK and nosZ genes were not affected. Watering significantly affected the ammonia-oxidizing archaea (AOA) community and N addition showed a strong effect on soil ammonia-oxidizing bacteria (AOB) and nosZ-containing denitrifier community structure. No significant interactive effects of mowing, N addition and watering on communities of soil ammonia oxidizers and denitrifiers were observed. Structure equation models suggested that plant biomass and community were significantly correlated with microbial communities and activities. These findings suggest that there are complex interactions between plants and soil microorganisms in grassland ecosystems.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/40576]
专题	生态环境研究中心_城市与区域生态国家重点实验室
推荐引用方式 GB/T 7714	He, Ji-Zheng,Zhang, Cui-Jing,Yang, Zhong-Ling,et al. Impacts of long-term nitrogen addition, watering and mowing on ammonia oxidizers, denitrifiers and plant communities in a temperate steppe[J]. APPLIED SOIL ECOLOGY,2018,130:241-250.
APA	He, Ji-Zheng.,Zhang, Cui-Jing.,Yang, Zhong-Ling.,Shen, Ju-Pei.,Sun, Yi-Fei.,...&Zhang, Li-Mei.(2018).Impacts of long-term nitrogen addition, watering and mowing on ammonia oxidizers, denitrifiers and plant communities in a temperate steppe.APPLIED SOIL ECOLOGY,130,241-250.
MLA	He, Ji-Zheng,et al."Impacts of long-term nitrogen addition, watering and mowing on ammonia oxidizers, denitrifiers and plant communities in a temperate steppe".APPLIED SOIL ECOLOGY 130(2018):241-250.