Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b
	Yin, Jiqing; Liu, Wenqiang; Jiang, Yonghua; Chen, Mo; Xu, Ruimin; Le, Rongrong; Li, Chong; Wang, Hong; Wan, Xiaoping; Wang, Hailin
刊名	STEM CELL REPORTS
	2018-02-13
卷号	10 期号:2 页码:477-493
ISSN号	2213-6711
文献子类	Article
英文摘要	Androgenetic haploid embryonic stem cells (AG-haESCs) hold great promise for exploring gene functions and generating gene-edited semi-cloned (SC) mice. However, the high incidence of self-diploidization and low efficiency of SC mouse production are major obstacles preventing widespread use of these cells. Moreover, although SC mice generation could be greatly improved by knocking out the differentially methylated regions of two imprinted genes, 50% of the SC mice did not survive into adulthood. Here, we found that the genome-wide DNA methylation level in AG-haESCs is extremely low. Subsequently, downregulation of both de novo methyltransferase Dnmt3b and other methylation-related genes was determined to be responsible for DNA hypomethylation. We further demonstrated that ectopic expression of Dnmt3b in AG-haESCs could effectively improve DNA methylation level, and the high incidence of self-diploidization could be markedly rescued. More importantly, the developmental potential of SC embryos was improved, and most SC mice could survive into adulthood.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/41026]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Yin, Jiqing,Liu, Wenqiang,Jiang, Yonghua,et al. Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b[J]. STEM CELL REPORTS,2018,10(2):477-493.
APA	Yin, Jiqing.,Liu, Wenqiang.,Jiang, Yonghua.,Chen, Mo.,Xu, Ruimin.,...&Zhao, Yanhong.(2018).Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b.STEM CELL REPORTS,10(2),477-493.
MLA	Yin, Jiqing,et al."Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b".STEM CELL REPORTS 10.2(2018):477-493.