High-performance Liquid Chromatography for the Quantification of Fructose-1,6 Diphosphate in Human Renal Epithelial Cells

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	High-performance Liquid Chromatography for the Quantification of Fructose-1,6 Diphosphate in Human Renal Epithelial Cells
	Zhu, Huiyu; Wang, Hailin
刊名	BOLETIN DE MALARIOLOGIA Y SALUD AMBIENTAL
	2018
卷号	58 期号:1 页码:20-25
关键词	HPLC Intracellular 293T Quantification F-1,6-DP
ISSN号	1690-4648
文献子类	Article
英文摘要	Fructose-1,6Diphosphate is an energy metabolism intermediate, and in recent years, it has shown many important functions in cells. So, it is necessary to develop methods for the quantification of Fructose-1,6Diphosphate in cells. A high-performance liquid chromatography (HPLC) method is developed in this study for analysis of the intracellular intermediate. This study provides a way to have an insight into the content of Fructose-1,6-Diphosphate in Human renal epithelial cell line 293T cells. An Angela Venusil MP C18 column and isocratic elution are adopted. Mobile phase A: 50 mM ammonium hydroxide, mobile phase B: 100% methanol. Isocratic elution and 5% methanol, the linear range is 0.1 similar to 50. M. LOD (S/N. 3) is 0.0001. M, and LOQ (S/N. 10) is 0.01. M. The fructose-1,6diphosphate in human renal epithelial cell line 293T cells is within the liner range. The developed method in this study is applied to determine fructose-1,6-diphosphate in human renal epithelial cell line 293T cells successfully.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/40953]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zhu, Huiyu,Wang, Hailin. High-performance Liquid Chromatography for the Quantification of Fructose-1,6 Diphosphate in Human Renal Epithelial Cells[J]. BOLETIN DE MALARIOLOGIA Y SALUD AMBIENTAL,2018,58(1):20-25.
APA	Zhu, Huiyu,&Wang, Hailin.(2018).High-performance Liquid Chromatography for the Quantification of Fructose-1,6 Diphosphate in Human Renal Epithelial Cells.BOLETIN DE MALARIOLOGIA Y SALUD AMBIENTAL,58(1),20-25.
MLA	Zhu, Huiyu,et al."High-performance Liquid Chromatography for the Quantification of Fructose-1,6 Diphosphate in Human Renal Epithelial Cells".BOLETIN DE MALARIOLOGIA Y SALUD AMBIENTAL 58.1(2018):20-25.