Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach
	Yan, Xueting; He, Bin; Liu, Lihong; Qu, Guangbo; Shi, Jianbo; Hu, Ligang; Jiang, Guibin
刊名	METALLOMICS
	2018-04-01
卷号	10 期号:4 页码:557-564
ISSN号	1756-5901
文献子类	Article
英文摘要	Silver nanoparticles (AgNPs) are the nanomaterials most widely used as antimicrobial agents in a range of consumer products, due to the environmental release of either the AgNPs themselves or silver ions. Although AgNPs appear to be more potent than silver ions, the mechanism behind the activity is not fully elucidated yet. The most common mechanism of toxicity of AgNPs proposed to date is the release of silver ions and/or the particle-specific functions. In this study, Pseudomonas aeruginosa (a model for Gram-negative bacteria) was treated with AgNPs, and its proteomic response was comprehensively characterized to elucidate the antimicrobial mechanism of AgNPs in the microorganism. In total, 59 silver-regulated proteins (27 up-regulated and 32 down-regulated proteins) and 5 silver-binding proteins were identified. Bioinformatic analysis revealed that interference with the cell-membrane function and generation of intracellular reactive oxygen species (ROS) were the main pathways for the antibacterial effect. The pattern of membrane proteins regulated by AgNPs was similar to that found for silver ions. In addition, the same silver-binding proteins were obtained with both AgNPs and silver ions, which indicated that AgNPs probably affect the cell membrane and react with proteins by releasing silver ions. The elevation of intracellular ROS relative to that with silver ions confirmed oxidative damage caused by AgNPs, which may be ascribed to the nano-characteristics and higher uptake efficiency of the particles. These results demonstrate that the antimicrobial activity of AgNPs is due to the synergistic action of release of dissolved silver ions and particle-specific effects. The proteomic analysis of silver-binding and silver-regulated proteins in the present study provides insight into the mechanism of antimicrobial activity of such nanomaterials.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/40877]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Yan, Xueting,He, Bin,Liu, Lihong,et al. Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach[J]. METALLOMICS,2018,10(4):557-564.
APA	Yan, Xueting.,He, Bin.,Liu, Lihong.,Qu, Guangbo.,Shi, Jianbo.,...&Jiang, Guibin.(2018).Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach.METALLOMICS,10(4),557-564.
MLA	Yan, Xueting,et al."Antibacterial mechanism of silver nanoparticles in Pseudomonas aeruginosa: proteomics approach".METALLOMICS 10.4(2018):557-564.