Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy

CORC > 沈阳自动化研究所 > 中国科学院沈阳自动化研究所 > 机器人学研究室

	Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy
	Hou, Jing 2; Jiao ND(焦念东)1 ; Liu LQ(刘连庆)1 ; Chen, Liangliang 8; Basson, Marc D.5,6; Dong, Lixin 8; Yang, Ruiguo 3; Xi N(席宁)4,8 ; Yang YL(杨永良)7,8; Zeng, Bixi 5,6
刊名	IEEE TRANSACTIONS ON NANOTECHNOLOGY
	2019
卷号	18 页码:509-517
关键词	Bionanotechnology nanosensors force spectroscopy atomic force microscopy protein-protein interactions
ISSN号	1536-125X
产权排序	8
英文摘要	Increasingly targeted in drug discovery, protein-protein interactions challenge current high throughput screening technologies in the pharmaceutical industry. Developing an effective and efficient method for screening small molecules or compounds is critical to accelerate the discovery of ligands for enzymes, receptors, and other pharmaceutical targets. Here, we report developments of methods to increase the signal-to-noise ratio for screening protein-protein interactions using atomic force microscopy (AFM) force spectroscopy. We have demonstrated the effectiveness of these developments on detecting the binding process between focal adhesion kinases with protein kinase B (Akt1), which is a target for potential cancer drugs. These developments include optimized probe and substrate functionalization processes and redesigned probe-substrate contact regimes. Furthermore, a statistical-based data processing method was developed to enhance the contrast of the experimental data. Collectively, these results demonstrate the potential of the AFM force spectroscopy in automating drug screening with high throughput.
语种	英语
WOS记录号	WOS:000469366400001
资助机构	Nebraska Center for Integrated Biomolecular Communication (NIH National Institutes of General Medical Sciences) [P20 GM113126] ; Nebraska Center for Nanomedicine [P30 GM127200] ; NSF [1826135]
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/24733]
专题	沈阳自动化研究所_机器人学研究室
通讯作者	Yang, Ruiguo; Xi N(席宁)
作者单位	1.e State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110006, China 2.School of Information and Control Engineering, Shenyang Jianzhu University, Shenyang 110168, China 3.Department of Mechanicaland Materials Engineering, University of Nebraska Lincoln, NE 68588 USA 4.Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong, Hong Kong, China 5.Department of Biomedical Sciences, University of North Dakota, Grand Forks, ND 58202 USA 6.Department of Surgery, University of North Dakota, Grand Forks, ND 58202 USA 7.Department of Biomedical Engineering, Michigan State University, East Lansing, MI 48823 USA 8.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48823 USA
推荐引用方式 GB/T 7714	Hou, Jing,Jiao ND,Liu LQ,et al. Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy[J]. IEEE TRANSACTIONS ON NANOTECHNOLOGY,2019,18:509-517.
APA	Hou, Jing.,Jiao ND.,Liu LQ.,Chen, Liangliang.,Basson, Marc D..,...&Esfahani, Amir Monemian.(2019).Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy.IEEE TRANSACTIONS ON NANOTECHNOLOGY,18,509-517.
MLA	Hou, Jing,et al."Optimization of Protein-Protein Interaction Measurements for Drug Discovery Using AFM Force Spectroscopy".IEEE TRANSACTIONS ON NANOTECHNOLOGY 18(2019):509-517.