Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei

doi:10.1016/j.fsi.2018.12.073

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei
	Du, Yang 1,2; Zhou, Shuhong 2,3; Liu, Mei 1,2; Wang, Baojie 1,2; Jiang, Keyong 1,2; Fang, Han 2,3; Wang, Lei 1,2,4
刊名	FISH & SHELLFISH IMMUNOLOGY
	2019-03-01
卷号	86 页码:1194-1206
关键词	Litopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins Immune response Gut bacteria diversity
ISSN号	1050-4648
DOI	10.1016/j.fsi.2018.12.073
通讯作者	Wang, Lei(wanglei@qdio.ac.cn)
英文摘要	The interactions of microbiota in the intestines play an important role in promoting or maintaining the health of hosts. The present study aim to investigate the effects of the surface proteins of Lactobacillus pentosus HC-2 on the immune response and the bacterial composition of Litopenaeus vannamei, thus, the immune -related genes, surface condition, HC-2 numbers and the bacteria diversity in midgut were explored after shrimp feeding the normal HC-2 and 5 M - lithium chloride (LiCI) treated HC-2 for four weeks. Obvious improvements in the intestinal surface were observed in R group than the control group and L group. qPCR analysis demonstrated that the selected immune -related genes of lysozyme, proPO, LGBP, PEN-3 alpha, crustin, and lvLec were significantly up-regulated in group R than in group L Meanwhile, in the challenge test, shrimp in R group received 72% relative percent survival, which was significantly higher than the L group (RPS = 9%). The bacteria composition analysis showed that the abundance of Proteobacteria were significantly higher in group R and L than in group C, and the Bacteroidetes were significantly higher in group C than in group R and L, whereas the numbers of Chloroflexi were significantly higher in group R than in group C and L. The bacterial community difference analysis revealed that the harmful bacteria such as genus of Vibrio, Tenacibaculu and Thalassobius were decreased and the beneficial bacterium as Ruegeria and Lactobacillus were increased in R group, whereas this phenomenon were not found in L group. Taken together, above results indicating that the surface proteins were indispensable for L. pentosus HC-2 adhesion and colonization in shrimp intestines to improve intestine condition, enhance immune response, competitively exclude the pathogens, and promote the beneficial bacteria growth to protect the shrimp from pathogens infection. The findings in this work will help to promote the understanding of the roles of probiotics in shrimp intestines displaying probiotic-function by regulating the intestinal bacteria.
资助项目	National Natural Science Foundation of China[31802309] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH] ; China Postdoctoral Science Foundation[2018M632736]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000459528400132
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/155394]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Peoples R China
推荐引用方式 GB/T 7714	Du, Yang,Zhou, Shuhong,Liu, Mei,et al. Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:1194-1206.
APA	Du, Yang.,Zhou, Shuhong.,Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,...&Wang, Lei.(2019).Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,86,1194-1206.
MLA	Du, Yang,et al."Understanding the roles of surface proteins in regulation of Lactobacillus pentosus HC-2 to immune response and bacterial diversity in midgut of Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 86(2019):1194-1206.