Physicochemical Characteristics and Anticoagulant Activities of the Polysaccharides from Sea Cucumber Pattalus mollis

doi:10.3390/md17040198

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Physicochemical Characteristics and Anticoagulant Activities of the Polysaccharides from Sea Cucumber Pattalus mollis
	Zheng, Wenqi 1; Zhou, Lutan 2,3; Lin, Lisha 2,3; Cai, Ying 2,3; Sun, Huifang 2,3; Zhao, Longyan1 ; Gao, Na 1; Yin, Ronghua 2; Zhao, Jinhua1,2
刊名	MARINE DRUGS
	2019-04-01
卷号	17 期号:4 页码:15
关键词	Pattalus mollis fucosylated glycosaminoglycan fucan sulfate physicochemical characteristics anticoagulant activities
ISSN号	1660-3397
DOI	10.3390/md17040198
通讯作者	Yin, Ronghua(yinronghua@mail.kib.ac.cn) ; Zhao, Jinhua(zhao.jinhua@yahoo.com)
英文摘要	Sulfated polysaccharides from sea cucumbers possess distinct chemical structure and various biological activities. Herein, three types of polysaccharides were isolated and purified from Pattalus mollis, and their structures and bioactivities were analyzed. The fucosylated glycosaminoglycan (PmFG) had a CS-like backbone composed of the repeating units of {-4-d-GlcA--1,3-d-GalNAc(4S6S)--1-}, and branches of a sulfated -l-Fuc (including Fuc(2S4S), Fuc(3S4S) and Fuc(4S) with a molar ratio of 2:2.5:1) linked to O-3 of each d-GlcA. The fucan sulfate (PmFS) had a backbone consisting of a repetitively linked unit {-4-l-Fuc(2S)--1-}, and interestingly, every trisaccharide unit in its backbone was branched with a sulfated -l-Fuc (Fuc(4S) or Fuc(3S) with a molar ratio of 4:1). Apart from the sulfated polysaccharides, two neutral glycans (PmNG-1 & -2) differing in molecular weight were also obtained and their structures were similar to animal glycogen. Anticoagulant assays indicated that PmFG and PmFS possessed strong APTT prolonging and intrinsic factor Xase inhibition activities, and the sulfated -l-Fuc branches might contribute to the anticoagulant and anti-FXase activities of both PmFG and PmFS.
WOS研究方向	Pharmacology & Pharmacy
语种	英语
WOS记录号	WOS:000467307100005
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/66911]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Yin, Ronghua; Zhao, Jinhua
作者单位	1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Wenqi,Zhou, Lutan,Lin, Lisha,et al. Physicochemical Characteristics and Anticoagulant Activities of the Polysaccharides from Sea Cucumber Pattalus mollis[J]. MARINE DRUGS,2019,17(4):15.
APA	Zheng, Wenqi.,Zhou, Lutan.,Lin, Lisha.,Cai, Ying.,Sun, Huifang.,...&Zhao, Jinhua.(2019).Physicochemical Characteristics and Anticoagulant Activities of the Polysaccharides from Sea Cucumber Pattalus mollis.MARINE DRUGS,17(4),15.
MLA	Zheng, Wenqi,et al."Physicochemical Characteristics and Anticoagulant Activities of the Polysaccharides from Sea Cucumber Pattalus mollis".MARINE DRUGS 17.4(2019):15.