Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system

doi:10.1016/j.carbpol.2016.01.011

	Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system
	Zheng, Huizhen 1,2; Gao, Meng 1,2; Song, Huiyi 1,2; Ren, Ying 1,2; Li, Shen 1,2; Liu, Xiudong 3; Yu, Weiting 2; Ma, Xiaojun 2
刊名	CARBOHYDRATE POLYMERS
	2016-05-05
卷号	141 页码:160-165
关键词	Alginate-chitosan Microencapsulation Entrapped Low-density Cells With Culture Quorum Sensing Viability
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2016.01.011
文献子类	Article
英文摘要	Entrapped low density cells with culture (ELDCwc) have been proved as a more effective way than direct entrapped high density cells (dEHDC) and free cells to protect probiotics from harsh environment, that is, to improve their stress resistance. The aim of this study was to investigate whether bacterial quorum sensing (QS) facilitated the stress resistance of Escherichia coli in microcapsules by detecting the expression of luxS/AI-2 system. As a result, both the expression of luxS gene and the concentration of autoinducer-2 (AI-2, QS signal molecule) have been discovered higher in ELDCwc than in dEHDC and free cells. Besides that, the luxS mutant E. coli strain was used as a negative control of QS to verify the influence of QS on bacterial stress resistance in microcapsules. The significantly decreased viability of luxS mutant strain in simulated gastric fluid also indicated that the QS played a critical role in protecting microorganisms from severe environment. (C) 2016 Elsevier Ltd. All rights reserved.
WOS关键词	LACTOBACILLUS-RHAMNOSUS ; ESCHERICHIA-COLI ; SALMONELLA-TYPHIMURIUM ; MICROENCAPSULATION ; SURVIVAL ; PROBIOTICS ; MICROENVIRONMENT ; THERMOTOLERANCE ; ENCAPSULATION ; CEREVISIAE
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000369960400019
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/171216]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu, Xiudong; Yu, Weiting
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 3.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Huizhen,Gao, Meng,Song, Huiyi,et al. Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system[J]. CARBOHYDRATE POLYMERS,2016,141:160-165.
APA	Zheng, Huizhen.,Gao, Meng.,Song, Huiyi.,Ren, Ying.,Li, Shen.,...&Ma, Xiaojun.(2016).Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system.CARBOHYDRATE POLYMERS,141,160-165.
MLA	Zheng, Huizhen,et al."Culture of low density E. coli cells in alginate chitosan microcapsules facilitates stress resistance by up-regulating luxS/AI-2 system".CARBOHYDRATE POLYMERS 141(2016):160-165.