Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules

doi:10.1016/j.jbiosc.2015.08.010

	Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules
	Ma, Xiaojun 1; Yu, Weiting 1; Gao, Meng 1,3; Song, Huiyi 1,3; Liu, Xiudong 2
刊名	JOURNAL OF BIOSCIENCE AND BIOENGINEERING
	2016-04-01
卷号	121 期号:4 页码:406-412
关键词	Quorum Sensing Entrapped Low Density Cells With Culture Cell Aggregates Microencapsulation Vibrio Harveyi
ISSN号	1389-1723
DOI	10.1016/j.jbiosc.2015.08.010
文献子类	Article
英文摘要	Microcapsule entrapped low density cells with culture (ELDCwc), different from free cell culture, conferred stronger stress resistance and improved cell viability of microorganisms. In this paper, the quorum sensing (QS) system of Vibrio harveyi was used to investigate changes when cells were cultured in microcapsules. Cells in ELDCwc group grew into cell aggregates, which facilitated cell-cell communication and led to increased bioluminescence intensity. Moreover, the luxS-AI-2 system, a well-studied QS signal pathway, was detected as both luxS gene and the AI-2 signaling molecule, and the results were analyzed with respect to QS capacity of unit cell. The V. harveyi of ELDCwc also showed higher relative gene expression and stronger quorum sensing capacity when compared with free cells. In conclusion, the confined microcapsule space can promote the cell aggregates formation, reduce cell-cell communication distance and increase local concentration of signal molecule, which are beneficial to bacterial QS. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.
WOS关键词	PROBIOTIC BACTERIA ; ALGINATE ; MICROENCAPSULATION ; EXPRESSION ; STRESS ; GENES ; CELLS ; MICROENVIRONMENT ; HETEROGENEITY ; PERMEABILITY
WOS研究方向	Biotechnology & Applied Microbiology ; Food Science & Technology
语种	英语
出版者	SOC BIOSCIENCE BIOENGINEERING JAPAN
WOS记录号	WOS:000375161900009
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170842]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yu, Weiting
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Univ, Coll Environm & Chem Engn, Dalian Econ Technol Dev Zone, Dalian 116622, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ma, Xiaojun,Yu, Weiting,Gao, Meng,et al. Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2016,121(4):406-412.
APA	Ma, Xiaojun,Yu, Weiting,Gao, Meng,Song, Huiyi,&Liu, Xiudong.(2016).Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,121(4),406-412.
MLA	Ma, Xiaojun,et al."Improved quorum sensing capacity by culturing Vibrio harveyi in microcapsules".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 121.4(2016):406-412.