Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening

doi:10.1016/j.msec.2016.01.045

	Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening
	Chao, Ji-Ye 2,3; Ruan, Wen-Ding 2,3; Du, Feng-guang 2,3; Meng, Yue-Zhong 4; Wang, Duo 2,3; Wang, Jian-Zheng 1,2,3; Zhu, Yu-Xia 1,2,3; Ma, Hui-Chao 2,3; Chen, Si-Nan 2,3
刊名	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
	2016-05-01
卷号	62 页码:215-225
关键词	Three-dimensional Cell Culture Multi-cellular Tumor Spheroid (Mots) Spray-spinning Chitosan/collagen/alginate Scaffold (Cca)
ISSN号	0928-4931
DOI	10.1016/j.msec.2016.01.045
文献子类	Article
英文摘要	In this work, a 3D MCTS-CCA system was constructed by culturing multi-cellular tumor spheroid (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening. The CCA scaffolds were fabricated by spray-spinning. The interactions between the components of the spray-spun fibers were evidenced by methods of Coomassie Blue stain, X-ray diffraction (XRD) and Fourier transform-infrared spectroscopy (FTIR). Co-culture indicated that MCF-7 cells showed a spatial growth pattern of multi-cellular tumor spheroid (MCTS) in the CCA fibrous scaffold with increased proliferation rate and drug-resistance to MMC, ADM and 5-Aza comparing with the 2D culture cells. Significant increases of total viable cells were found in 3D MCTS groups after drug administration by method of apoptotic analysis. Glucose-lactate analysis indicated that the metabolism of MCTS in CCA scaffold was closer to the tumor issue in vivo than the monolayer cells. In addition, MCTS showed the characteristic of epithelial mesenchymal transition (EMT) which is subverted by carcinoma cells to facilitate metastatic spread. These results demonstrated that MCTS in CCA scaffold possessed a more conservative phenotype of tumor than monolayer cells, and anticancer drug screening in 3D MCTS-CCA system might be superior to the 2D culture system. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词	MESENCHYMAL STEM-CELLS ; CANCER-CELL ; MULTICELLULAR SPHEROIDS ; CHITOSAN-ALGINATE ; MAMMALIAN TARGET ; EPITHELIAL-CELLS ; BREAST-CANCER ; MASS-TRANSFER ; CULTURE ; BONE
WOS研究方向	Materials Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000372759100027
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170642]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wang, Jian-Zheng
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Tianguan Grp Co Ltd, Affiliated Gen Hosp, Nanyang 473000, Peoples R China 3.Testing Ctr Henan Tianguan Grp Co Ltd, 1 Tianguan Rd, Nanyang 473000, Henan Province, Peoples R China 4.Sun Yat Sen Univ, State Key Lab Optoelect Mat & Technol, Guangzhou 510275, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Chao, Ji-Ye,Ruan, Wen-Ding,Du, Feng-guang,et al. Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2016,62:215-225.
APA	Chao, Ji-Ye.,Ruan, Wen-Ding.,Du, Feng-guang.,Meng, Yue-Zhong.,Wang, Duo.,...&Chen, Si-Nan.(2016).Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,62,215-225.
MLA	Chao, Ji-Ye,et al."Developing multi-cellular tumor spheroid model (MOTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 62(2016):215-225.