Human induced pluripotent stem cells derived endothelial cells mimicking vascular inflammatory response under flow

doi:10.1063/1.4940041

	Human induced pluripotent stem cells derived endothelial cells mimicking vascular inflammatory response under flow
	Xiang, Meng 1; Wang, Li 1,2; Liu, Yingying 1; Sun, Ning 1; Lu, Meng 1; Shi, Yang 2; Wang, Xinhong 1; Meng, Dan 1; Chen, Sifeng 1; Qin, Jianhua 2
刊名	BIOMICROFLUIDICS
	2016
卷号	10 期号:1
ISSN号	1932-1058
DOI	10.1063/1.4940041
文献子类	Article
英文摘要	Endothelial cells (ECs) have great potential in vascular diseases research and regenerative medicine. Autologous human ECs are difficult to acquire in sufficient numbers in vitro, and human induced pluripotent stem cells (iPSCs) offer unique opportunity to generate ECs for these purposes. In this work, we present a new and efficient method to simply differentiate human iPSCs into functional ECs, which can respond to physiological level of flow and inflammatory stimulation on a fabricated microdevice. The endothelial-like cells were differentiated from human iPSCs within only one week, according to the inducing development principle. The expression of endothelial progenitor and endothelial marker genes (GATA2, RUNX1, CD34, and CD31) increased on the second and fourth days after the initial inducing process. The differentiated ECs exhibited strong expression of cells-specific markers (CD31 and von Willebrand factor antibody), similar to that present in human umbilical vein endothelial cells. In addition, the hiPSC derived ECs were able to form tubular structure and respond to vascular-like flow generated on a microdevice. Furthermore, the human induced pluripotent stem cell-endothelial cells (hiPSC-ECs) pretreated with tumor necrosis factor (TNF-alpha) were susceptible to adhesion to human monocyte line U937 under flow condition, indicating the feasibility of this hiPSCs derived microsystem for mimicking the inflammatory response of endothelial cells under physiological and pathological process. (C) 2016 AIP Publishing LLC.
WOS关键词	SHEAR-STRESS ; DIFFERENTIATION ; ANGIOGENESIS ; DYSFUNCTION ; COMMITMENT ; DERIVATION ; ARTERIAL ; DISEASE ; GATA2 ; MODEL
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Science & Technology - Other Topics ; Physics
语种	英语
出版者	AMER INST PHYSICS
WOS记录号	WOS:000377536500024
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170624]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Chen, Sifeng; Qin, Jianhua
作者单位	1.Fudan Univ, Coll Basic Med Sci, Dept Physiol & Pathophysiol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Xiang, Meng,Wang, Li,Liu, Yingying,et al. Human induced pluripotent stem cells derived endothelial cells mimicking vascular inflammatory response under flow[J]. BIOMICROFLUIDICS,2016,10(1).
APA	Xiang, Meng.,Wang, Li.,Liu, Yingying.,Sun, Ning.,Lu, Meng.,...&Qin, Jianhua.(2016).Human induced pluripotent stem cells derived endothelial cells mimicking vascular inflammatory response under flow.BIOMICROFLUIDICS,10(1).
MLA	Xiang, Meng,et al."Human induced pluripotent stem cells derived endothelial cells mimicking vascular inflammatory response under flow".BIOMICROFLUIDICS 10.1(2016).