The Crystal Structure of the YknZ Extracellular Domain of ABC Transporter YknWXYZ from Bacillus amyloliquefaciens

doi:10.1371/journal.pone.0155846

	The Crystal Structure of the YknZ Extracellular Domain of ABC Transporter YknWXYZ from Bacillus amyloliquefaciens
	Xu, Yongbin 3,4; Guo, Jianyun 3; Wang, Lulu 1; Jiang, Rui 2; Jin, Xiaoling 3; Liu, Jing 3; Fan, Shengdi 3; Quan, Chun-Shan 3; Ha, Nam-Chul 5
刊名	PLOS ONE
	2016-05-31
卷号	11 期号:5
ISSN号	1932-6203
DOI	10.1371/journal.pone.0155846
文献子类	Article
英文摘要	Bacillus possesses the peptide toxin Sporulation-Delaying Protein (SDP), which can kill cells within a biofilm to support continued growth, thereby delaying the onset of biofilm sporulation. The four-component transporter YknWXYZ acts as a major SDP efflux pump to protect cells against the endogenous SDP toxin, for which YknYZ is a non-canonical ATP-binding cassette (ABC)-type transporter. YknYZ consists of the following two components: (1) an individual protein (YknY) and (2) a respective permease (YknZ). To date, the crystal structure, molecular function, and mechanism of action of the integral membrane protein YknZ remain to be elucidated. In this study, to characterize the structural and biochemical roles of YknZ in the functional assembly of YknWXYZ, we predicted and overexpressed the YknZ extracellular domain. We determined the crystal structure of B. amyloliquefaciens YknZ at a resolution of 2.0 angstrom. The structure revealed that the YknZ extracellular region exhibits significant structural similarity with the MacB periplasmic domain, which is a non-canonical ABC-type transporter in the tripartite macrolide-specific efflux pump in Gram-negative bacteria. We also found that the YknZ extracellular domain can directly bind to an extracellular component of YknX. This structural and biochemical study provides insights into the assembly of YknWXYZ, which may be relevant to understanding cannibalistic peptide toxin resistance in Bacillus and controlling bacterial growth.
WOS关键词	ESCHERICHIA-COLI ; EFFLUX PUMP ; SUBTILIS ; MODEL ; MACB ; TOPOLOGY ; TOOLS
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000377146100009
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170580]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xu, Yongbin
作者单位	1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Liaoning, Peoples R China 2.Northeastern Univ, Coll Life & Hlth Sci, Shenyang 110004, Liaoning, Peoples R China 3.Dalian Nationalities Univ, Coll Life Sci, Dept Bioengn, Dalian 116600, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Liaoning, Peoples R China 5.Seoul Natl Univ, Dept Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
推荐引用方式 GB/T 7714	Xu, Yongbin,Guo, Jianyun,Wang, Lulu,et al. The Crystal Structure of the YknZ Extracellular Domain of ABC Transporter YknWXYZ from Bacillus amyloliquefaciens[J]. PLOS ONE,2016,11(5).
APA	Xu, Yongbin.,Guo, Jianyun.,Wang, Lulu.,Jiang, Rui.,Jin, Xiaoling.,...&Ha, Nam-Chul.(2016).The Crystal Structure of the YknZ Extracellular Domain of ABC Transporter YknWXYZ from Bacillus amyloliquefaciens.PLOS ONE,11(5).
MLA	Xu, Yongbin,et al."The Crystal Structure of the YknZ Extracellular Domain of ABC Transporter YknWXYZ from Bacillus amyloliquefaciens".PLOS ONE 11.5(2016).