Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy

doi:10.1016/j.chroma.2016.07.042

	Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy
	Liu, Zheyi 1,2; Wang, Fangjun 2; Chen, Jin 1,2; Zhou, Ye 1,2; Zou, Hanfa 2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2016-08-26
卷号	1461 页码:35-41
关键词	Multi-phosphopeptide Enrichment Competitive Substitution Pre-coordination Mass Spectrometry Phosphoproteomics
ISSN号	0021-9673
DOI	10.1016/j.chroma.2016.07.042
文献子类	Article
英文摘要	Although many affinity adsorbents have been developed for phosphopeptides enrichment, high specifically capturing the multi-phosphopeptides is still a big challenge. Here, we investigated the mechanism of phosphate ion coordination and substitution on affinity adsorbents surfaces and modulated the selectivity of affinity adsorbents to multi-phosphopeptides based on the different capability of mono and multi-phosphopeptides in competitively substituting the pre-coordinated phosphate ions at strong acidic condition. We demonstrated both the species of pre-coordinated phosphate ions and the substituting conditions played crucial roles in modulating the enrichment selectivity to multi-phosphopeptides, and the pre-coordinated affinity materials with relative more surfaces positive charges exhibited better enrichment efficiency due to the cooperative effect of electrostatic interaction and competitive substitution. Finally, an enrichment selectivity of 85% to multi-phosphopeptides was feasibly achieved with 66% improvement in identification numbers for complex protein sample extracted from HepG2 cells. Data are available via ProteomeXchange with identifier PXD004252. (c) 2016 Elsevier B.V. All rights reserved.
WOS关键词	PHOSPHORYLATED PEPTIDES ; PROTEIN-PHOSPHORYLATION ; MASS-SPECTROMETRY ; TITANIUM-DIOXIDE ; ENRICHMENT ; PHOSPHOPROTEOME ; CHROMATOGRAPHY ; SEPARATION ; NETWORKS ; TIO2
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000381840200005
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170300]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wang, Fangjun
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zheyi,Wang, Fangjun,Chen, Jin,et al. Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1461:35-41.
APA	Liu, Zheyi,Wang, Fangjun,Chen, Jin,Zhou, Ye,&Zou, Hanfa.(2016).Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy.JOURNAL OF CHROMATOGRAPHY A,1461,35-41.
MLA	Liu, Zheyi,et al."Modulating the selectivity of affinity absorbents to multi-phosphopeptides by a competitive substitution strategy".JOURNAL OF CHROMATOGRAPHY A 1461(2016):35-41.