Label-Free Cell Phenotypic Assays for Assessing Drug Polypharmacology

doi:10.2174/1381612822666160224142048

	Label-Free Cell Phenotypic Assays for Assessing Drug Polypharmacology
	Wang, Jixia 1; Zhang, Xiuli 1; Fang, Ye 2; Liang, Xinmiao 1,3
刊名	CURRENT PHARMACEUTICAL DESIGN
	2016
卷号	22 期号:21 页码:3190-3200
关键词	Label-free Cell Phenotypic Assay Mechanism Of Action Multi-target Screen Polypharmacology Resonant Waveguide Grating Biosensor Electric Biosensor
ISSN号	1381-6128
DOI	10.2174/1381612822666160224142048
文献子类	Article
英文摘要	Background: Most drugs exert their biological and physiological effects via binding to protein targets. Although drugs are traditionally optimized against a single protein, most marketed drugs exhibit clinically relevant polypharmacology - the activity of drugs at multiple targets. The wide-spread presence of polypharmacology makes it challenging to assess the mechanisms of action of multi-target drugs. Methods: This paper first reviews approaches for discovering multi-targets of drug molecules, then discusses key characteristics of label-free cell phenotypic assays, and finally focuses on how to use these assays to assess drug polypharmacology. Results: label-free cell phenotypic assays have ability to provide a holistic view of drug action in living cells with wide phenotype/target/pathway coverage, and permit effective deconvolution of the action of multi-target drugs at the whole cell level. Conclusion: Label-free cell phenotypic assays hold great potential in assessing drug polypharmacology.
WOS关键词	DYNAMIC MASS REDISTRIBUTION ; PHARMACOLOGY ON-TARGET ; IMPEDANCE SENSING ECIS ; FREE OPTICAL BIOSENSOR ; ADVANCED SOLID TUMORS ; REAL-TIME ; PHASE-I ; SMALL-MOLECULE ; BETA(2)-ADRENERGIC RECEPTOR ; KINASE INHIBITOR
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
WOS记录号	WOS:000382674200011
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/170048]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Fang, Ye; Liang, Xinmiao
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Liaoning, Peoples R China 2.Corning Inc, Div Sci & Technol, Biochem Technol, Corning, NY 14831 USA 3.Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226019, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jixia,Zhang, Xiuli,Fang, Ye,et al. Label-Free Cell Phenotypic Assays for Assessing Drug Polypharmacology[J]. CURRENT PHARMACEUTICAL DESIGN,2016,22(21):3190-3200.
APA	Wang, Jixia,Zhang, Xiuli,Fang, Ye,&Liang, Xinmiao.(2016).Label-Free Cell Phenotypic Assays for Assessing Drug Polypharmacology.CURRENT PHARMACEUTICAL DESIGN,22(21),3190-3200.
MLA	Wang, Jixia,et al."Label-Free Cell Phenotypic Assays for Assessing Drug Polypharmacology".CURRENT PHARMACEUTICAL DESIGN 22.21(2016):3190-3200.