Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications

doi:10.1016/j.carbpol.2016.08.098

	Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications
	Pan, Guoyu 4; Ma, Xiaojun 1; Lou, Ruyun 1,2; Yu, Weiting 1; Song, Yizhe 1,2; Ren, Ying 1,2; Zheng, Huizhen 1,2; Guo, Xin 1; Lin, Yunfei 4; Wang, Xiuli 3
刊名	CARBOHYDRATE POLYMERS
	2017-01-02
卷号	155 页码:456-465
关键词	Galactosylated Alginate Hydroxyl Groups Microcapsules Primary Hepatocytes Biological Artificial Liver Support Systems
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2016.08.098
文献子类	Article
英文摘要	Galactose moieties are covalently coupled with sodium alginate to enhance liver-specific functions in microcapsules owing to the specific interaction between the galactose moieties and the asialoglycoprotein receptors (ASGPRs) of hepatocytes. In this study, galactosylated alginate (L-NH2-OH-alginate) based microcapsules with desirable stability and a suitable 3D microenvironment are designed and fabricated for primary hepatocyte applications. The designed L-NH2-OH-alginate is fabricated via the application of ethylenediamine grafted lactobionic acid (L-NH2) onto the hydroxyl groups of sodium alginate so that the negatively charged carboxyl groups intact in L-NH2-OH-alginate can effectively bond with Ca2+ to form a stable three-dimensional gel network; a subsequent reaction with polycations forms a stable membrane of microcapsules. As a result, L-NH2-OH-alginate based microcapsules exhibit an excellent mechanical stability. Moreover, with a higher degree of substitution in L-NH2-OH-alginate (DS 0.41), the hepatocytes entrapped in L-NH2-OH-alginate microcapsules exhibit better viability and well-maintained liver-specific functions. (C) 2016 Elsevier Ltd. All rights reserved.
WOS关键词	MICROENCAPSULATED HEPATOCYTES ; MECHANICAL STABILITY ; EXTRACELLULAR-MATRIX ; BIOARTIFICIAL LIVER ; CULTURE ; CHITOSAN ; RAT ; ENCAPSULATION ; OPTIMIZATION ; SUBSTRATUM
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000386402600050
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169942]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yu, Weiting; Wang, Xiuli
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Med Univ, Dept Histol & Embryol, Coll Basic Med Sci, Dalian 116044, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Pan, Guoyu,Ma, Xiaojun,Lou, Ruyun,et al. Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications[J]. CARBOHYDRATE POLYMERS,2017,155:456-465.
APA	Pan, Guoyu.,Ma, Xiaojun.,Lou, Ruyun.,Yu, Weiting.,Song, Yizhe.,...&Wang, Xiuli.(2017).Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications.CARBOHYDRATE POLYMERS,155,456-465.
MLA	Pan, Guoyu,et al."Fabrication of stable galactosylated alginate microcapsules via covalent coupling onto hydroxyl groups for hepatocytes applications".CARBOHYDRATE POLYMERS 155(2017):456-465.