A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells

doi:10.1016/j.bios.2016.06.039

	A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells
	Li, Peng 2,3; Tian, Fengshou 1; Jia, Yan 2,3; Zhang, Yanan 1,2,3; Song, Wei 4; Zhao, Guangjiu 2,3; Qu, Zongjin 2,3; Li, Chunyan 1,2,3; Chen, Yahong 1
刊名	BIOSENSORS & BIOELECTRONICS
	2016-12-15
卷号	86 页码:68-74
关键词	Near-infrared Clo-/mpo Detection Bio-imaging Real-time Monitoring Mitochondria Targeting
ISSN号	0956-5663
DOI	10.1016/j.bios.2016.06.039
文献子类	Article
英文摘要	Hyperchlorous acid (HClO), produced from MPO, is recognized as a host defense that kills pathogens; a signaling molecule that initiates cell apoptosis; and a harmful agent when overproduced. Thus, measuring of endogenous HClO and MPO will always find its great importance in revealing biological roles under complex biological conditions. In this study, a turn-on near infrared (NIR) fluorescent probe Cy7-NphS has been designed and developed for highly selective and sensitive sensing of HClO and Myeloperoxidase (MPO) with fast response time. The newly developed probe has been successfully applied in real-time monitoring of HClO and MPO activity in PBS solutions and living HL-60 cells. When applied in MPO activity determination, the probe showed very high sensitivity with a detection limit of as low as 3.69 x 10(-3) U/mL. Furthermore, the living cell imaging study suggested that this probe could detect HClO in mitochondria. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词	HYPOCHLOROUS ACID ; SALICYLHYDROXAMIC ACID ; GENERATED OXIDANTS ; IN-VIVO ; OXIDATION ; INFLAMMATION ; INVOLVEMENT ; INHIBITION ; BODIPY ; DESIGN
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000384853300011
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169848]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Li, Peng
作者单位	1.Zhoukou Normal Univ, Dept Chem, Zhoukou 466001, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Dalian Med Univ, Affiliated Hosp 1, 222 Zhongshan Rd, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Li, Peng,Tian, Fengshou,Jia, Yan,et al. A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells[J]. BIOSENSORS & BIOELECTRONICS,2016,86:68-74.
APA	Li, Peng.,Tian, Fengshou.,Jia, Yan.,Zhang, Yanan.,Song, Wei.,...&Chen, Yahong.(2016).A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells.BIOSENSORS & BIOELECTRONICS,86,68-74.
MLA	Li, Peng,et al."A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells".BIOSENSORS & BIOELECTRONICS 86(2016):68-74.