Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy

doi:10.1021/acs.analchem.6b02887

	Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy
	Chen, Rui; Qin, Hongqiang; Cheng, Kai; Zhu, Jun; Mao, Jiawei; Wang, Fangjun; Dong, Mingming; Guo, Zhimou; Liang, Xinmiao; Ye, Mingliang
刊名	ANALYTICAL CHEMISTRY
	2017-02-07
卷号	89 期号:3 页码:1469-1476
ISSN号	0003-2700
DOI	10.1021/acs.analchem.6b02887
文献子类	Article
英文摘要	The diversity of O-linked glycan structures has drawn increasing attention due to its vital biological roles. However, intact O-glycopeptides with different glycans are typically not well elucidated using the current methods. In this work, an integrated strategy was developed for comprehensive analysis of O-GalNAc glycosylation by combining hydrophilic interaction chromatography (HILIC) tip enrichment, beam-type collision induced decomposition (beam-CID) detection, and in silico deglycosylation method for spectra interpretation. In this strategy, the intact O-GalNAc glycopeptides were selectively enriched and the original spectra obtained by time-of-flight (TOF)-CID were preprocessed using an in silico deglycosylation method, enabling direct searching without setting multiple glycosylation modifications, which could significantly decrease the search space. This strategy was applied to analyze the O-GalNAc glycoproteome of human serum, leading to identification of 407 intact O-GalNAc glycopeptides from 93 glycoproteins. About 81% of the glycopeptides contained at least one sialic acid, which could reveal the microheterogeneity of O-GalNAc glycosylation. Up until now, this is the largest data set of intact O-GalNAc glycoforms from complex biological samples at the proteome level. Furthermore, this method is readily applicable to study O-glycoform heterogeneity in other complex biological systems.
WOS关键词	SITE-SPECIFIC CHARACTERIZATION ; HUMAN CEREBROSPINAL-FLUID ; SOLID-PHASE EXTRACTION ; LINKED GLYCOPEPTIDES ; MASS-SPECTROMETRY ; N-GLYCOSYLATION ; PLASMA-PROTEINS ; OVARIAN-CANCER ; LC-MS/MS ; IDENTIFICATION
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000393738300014
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169606]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Ye, Mingliang
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Chen, Rui,Qin, Hongqiang,Cheng, Kai,et al. Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy[J]. ANALYTICAL CHEMISTRY,2017,89(3):1469-1476.
APA	Chen, Rui.,Qin, Hongqiang.,Cheng, Kai.,Zhu, Jun.,Mao, Jiawei.,...&Zou, Hanfa.(2017).Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy.ANALYTICAL CHEMISTRY,89(3),1469-1476.
MLA	Chen, Rui,et al."Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy".ANALYTICAL CHEMISTRY 89.3(2017):1469-1476.