A novel fluorescent turn-on biosensor based on QDs@GSH-GO fluorescence resonance energy transfer for sensitive glutathione S-transferase sensing and cellular imaging | |
Chang, Lifang1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3 | |
刊名 | NANOSCALE |
2017 | |
卷号 | 9期号:11页码:3881-3888 |
ISSN号 | 2040-3364 |
DOI | 10.1039/c6nr09944k |
文献子类 | Article |
英文摘要 | A novel fluorescent turn-on biosensor based on fluorescence resonance energy transfer (FRET) from GSH functionalized Mn-doped ZnS QDs to graphene oxide (GO) was constructed to determine glutathione S-transferases (GSTs) in live cells and human urine. The QDs@GSH is adsorbed on the GO surface via hydrogen bonding interaction between the GSH on the surface of QDs@GSH and GO, and as a result, fluorescence quenching of the QDs@GSH takes place because of FRET. The FRET efficiency from QDs@GSH to GO was calculated to be 86.3%. However, in the presence of GSTs, the FRET process could be inhibited by the specific interaction between the GSH on the surface of QDs@GSH and GSTs, which would keep the QDs@GSH far away from the GO surface, leading to the recovery of the fluorescence. The proposed sensor exhibited high sensitivity, selectivity, and excellent specificity in the buffer, live cells and human urine for the detection of GSTs. Under the physiological conditions (pH 7.4), dissociation constants and the detection limit of GST and ATP6 V1F (a GST-tagged protein) were estimated to be 8.0 x 10(-9) M, 2.1 x 10(-10) M and 3.5 x 10(-9) M, 7.2 x 10(-11) M, respectively. The presented method has been successfully utilized for the determination of the GSTs in live cells and human urine without any complicated pretreatment and the recovery was in the range of 80%-90%. |
WOS关键词 | UP-CONVERSION NANOPARTICLES ; QUANTUM DOTS ; GRAPHENE OXIDE ; SEMICONDUCTOR NANOCRYSTALS ; FRET ; INHIBITORS ; AFFINITY ; PROBES ; BIOMOLECULES ; ARCHITECTURE |
WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
语种 | 英语 |
出版者 | ROYAL SOC CHEMISTRY |
WOS记录号 | WOS:000397126000022 |
内容类型 | 期刊论文 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169558] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Chen, Langxing |
作者单位 | 1.Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
推荐引用方式 GB/T 7714 | Chang, Lifang,He, Xiwen,Chen, Langxing,et al. A novel fluorescent turn-on biosensor based on QDs@GSH-GO fluorescence resonance energy transfer for sensitive glutathione S-transferase sensing and cellular imaging[J]. NANOSCALE,2017,9(11):3881-3888. |
APA | Chang, Lifang,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).A novel fluorescent turn-on biosensor based on QDs@GSH-GO fluorescence resonance energy transfer for sensitive glutathione S-transferase sensing and cellular imaging.NANOSCALE,9(11),3881-3888. |
MLA | Chang, Lifang,et al."A novel fluorescent turn-on biosensor based on QDs@GSH-GO fluorescence resonance energy transfer for sensitive glutathione S-transferase sensing and cellular imaging".NANOSCALE 9.11(2017):3881-3888. |
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