Chromatographic methods for the analysis of oligosaccharides in human milk

doi:10.1039/c6ay02982e

	Chromatographic methods for the analysis of oligosaccharides in human milk
	Yan, Jingyu 1; Liang, Xinmiao 1; Ding, Junjie 1,2
刊名	ANALYTICAL METHODS
	2017-02-21
卷号	9 期号:7 页码:1071-1077
ISSN号	1759-9660
DOI	10.1039/c6ay02982e
文献子类	Review
英文摘要	Human milk oligosaccharides (HMOs) are important components in human milk because of their high concentrations and benefits to infant growth. To better understand the relationship between oligosaccharide structures and biofunctions, efficient qualitative and quantitative methods are essential prerequisites. However, HMOs are highly complex and difficult to analyze because of their high polarity, lack of chromophores, and the presence of multiple isomeric structures. Although advanced mass spectrometry can provide much detailed structural information, chromatography is still important to reduce the complexity of HMOs in mixtures. The objective of this review is to describe and discuss the recent advances in the analysis of HMOs with the applications of different chromatographic methods, including gel permeation, high performance anion-exchange, porous graphitized carbon, hydrophilic interaction, reversed-phase high performance liquid chromatography, and capillary electrophoresis. These methods have been used to characterize the structures of HMOs, profile oligosaccharides obtained from the milk of different mothers, and measure the concentration changes in the course of lactations. For each method, we focus on the separation principles, commonly used separation conditions, typical applications, and the ability to resolve the isomers.
WOS关键词	HYDROPHILIC INTERACTION CHROMATOGRAPHY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; IONIZATION-MASS-SPECTROMETRY ; THIN-LAYER-CHROMATOGRAPHY ; SAMPLE PREPARATION METHOD ; CAPILLARY-ELECTROPHORESIS ; NEUTRAL OLIGOSACCHARIDES ; SIALYLATED OLIGOSACCHARIDES ; STRUCTURAL DETERMINATION ; H-1-NMR SPECTROSCOPY
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000395684800001
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169432]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liang, Xinmiao
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yan, Jingyu,Liang, Xinmiao,Ding, Junjie. Chromatographic methods for the analysis of oligosaccharides in human milk[J]. ANALYTICAL METHODS,2017,9(7):1071-1077.
APA	Yan, Jingyu,Liang, Xinmiao,&Ding, Junjie.(2017).Chromatographic methods for the analysis of oligosaccharides in human milk.ANALYTICAL METHODS,9(7),1071-1077.
MLA	Yan, Jingyu,et al."Chromatographic methods for the analysis of oligosaccharides in human milk".ANALYTICAL METHODS 9.7(2017):1071-1077.