Discovery and validation of potential urinary biomarkers for bladder cancer diagnosis using a pseudotargeted GC-MS metabolomics method

doi:10.18632/oncotarget.14988

	Discovery and validation of potential urinary biomarkers for bladder cancer diagnosis using a pseudotargeted GC-MS metabolomics method
	Xu, Chuanliang 3; Zhou, Yang 1,2; Song, Ruixiang 3; Ma, Chong 3; Sun, Yinghao 3; Xu, Guowang 1; Yin, Peiyuan 1; Zhou, Lina 1; Zhang, Zhensheng 3; Lu, Xin 1
刊名	ONCOTARGET
	2017
卷号	8 期号:13 页码:20719-20728
关键词	Metabolomics Gas Chromatography-mass Spectrometry Biomarker Urine Bladder Cancer
ISSN号	1949-2553
DOI	10.18632/oncotarget.14988
文献子类	Article
英文摘要	Bladder cancer (BC) is the second most prevalent malignancy in the urinary system and is associated with significant mortality; thus, there is an urgent need for novel noninvasive diagnostic biomarkers. A urinary pseudotargeted method based on gas chromatography-mass spectrometry was developed and validated for a BC metabolomics study. The method exhibited good repeatability, intraday and interday precision, linearity and metabolome coverage. A total of 76 differential metabolites were defined in the discovery sample set, 58 of which were verified using an independent validation urine set. The verified differential metabolites revealed that energy metabolism, anabolic metabolism and cell redox states were disordered in BC. Based on a binary logistic regression analysis, a four-biomarker panel was defined for the diagnosis of BC. The area under the receiving operator characteristic curve was 0.885 with 88.0% sensitivity and 85.7% specificity in the discovery set and 0.804 with 78.0% sensitivity and 70.3% specificity in the validation set. The combinatorial biomarker panel was also useful for the early diagnosis of BC. This approach can be used to discriminate non-muscle invasive and low-grade BCs from healthy controls with satisfactory sensitivity and specificity. The results show that the developed urinary metabolomics method can be employed to effectively screen noninvasive biomarkers.
WOS关键词	GAS CHROMATOGRAPHY/MASS SPECTROMETRY ; PENTOSE-PHOSPHATE PATHWAY ; MASS-SPECTROMETRY ; DECONVOLUTION ; METABOLISM
WOS研究方向	Oncology ; Cell Biology
语种	英语
出版者	IMPACT JOURNALS LLC
WOS记录号	WOS:000397642400020
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169394]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xu, Chuanliang; Lu, Xin
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Secondary Mil Med Univ, Shanghai Changhai Hosp, Dept Urol, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Xu, Chuanliang,Zhou, Yang,Song, Ruixiang,et al. Discovery and validation of potential urinary biomarkers for bladder cancer diagnosis using a pseudotargeted GC-MS metabolomics method[J]. ONCOTARGET,2017,8(13):20719-20728.
APA	Xu, Chuanliang.,Zhou, Yang.,Song, Ruixiang.,Ma, Chong.,Sun, Yinghao.,...&Liu, Xinyu.(2017).Discovery and validation of potential urinary biomarkers for bladder cancer diagnosis using a pseudotargeted GC-MS metabolomics method.ONCOTARGET,8(13),20719-20728.
MLA	Xu, Chuanliang,et al."Discovery and validation of potential urinary biomarkers for bladder cancer diagnosis using a pseudotargeted GC-MS metabolomics method".ONCOTARGET 8.13(2017):20719-20728.