DNA-Templated Aptamer Probe for Identification of Target Proteins

doi:10.1021/acs.analchem.6b04895

	DNA-Templated Aptamer Probe for Identification of Target Proteins
	Zhai, Guijin 2; Tian, Shanshan 2; Bi, Wenjing 2; Bai, Xue 2; Gao, Fan 1; Zhang, Kai 2; Zhang, Yukui 1,5; Lu, Congcong 1; Wang, Ye 1; Fan, Enguo 3,4
刊名	ANALYTICAL CHEMISTRY
	2017-04-04
卷号	89 期号:7 页码:4071-4076
ISSN号	0003-2700
DOI	10.1021/acs.analchem.6b04895
文献子类	Article
英文摘要	Using aptamers as molecular probes for biomarker discovery has attracted a great deal of attention in recent years. However, it is still a big challenge to accurately identify those protein markers that are targeted by aptamers under physiological conditions due to weak and noncovalent aptamer-protein interactions. Herein, we developed an aptamer based dual-probe using, DNA-templated chemistry and photo-cross-linking technique for the identification of target proteins that are recognized by aptamers. In this system, the aptamer was modified by a single strand DNA as binding probe (BP), and another complementary DNA with a photoactive group and reporter group was modified as capture probe (CP). BP was first added to recruit the binding protein via aptamer recognition, and Subsequently CP was added to let the cross-linker close to the target via DNA self-assembly, and then a covalent bond between CP and its binding protein was achieved via photo-cross-linking reaction. The captured protein can be detected or affinity enrichment using the tag, finally identified by MS. By use of lysozyme as a model substrate, we demonstrated that this multiple functionalized probe can be utilized for a successful labeling and enrichment of target protein even under a complicated and real environment. Thus, a novel method to precisely identify the aptamer-targeted proteins has been developed and it has a potential application for discovery of aptamer-based biomarkers.
WOS关键词	BINDING PROTEINS ; CANCER-CELLS ; RNA APTAMER ; SELECTION ; APTASENSOR ; ENRICHMENT ; LYSOZYME ; LIGANDS
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000398645300038
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169294]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhang, Kai
作者单位	1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Tianjin Med Univ, Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet, Key Lab Immune Microenvironm & Dis,Minist Educ, Tianjin 300070, Peoples R China 3.Univ Freiburg, Inst Biochem & Mol Biol, ZBMZ, Fac Med, D-79104 Freiburg, Germany 4.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Microbiol & Parasitol, Sch Basic Med,Peking Union Med Coll, Beijing 100005, Peoples R China 5.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhai, Guijin,Tian, Shanshan,Bi, Wenjing,et al. DNA-Templated Aptamer Probe for Identification of Target Proteins[J]. ANALYTICAL CHEMISTRY,2017,89(7):4071-4076.
APA	Zhai, Guijin.,Tian, Shanshan.,Bi, Wenjing.,Bai, Xue.,Gao, Fan.,...&Fan, Enguo.(2017).DNA-Templated Aptamer Probe for Identification of Target Proteins.ANALYTICAL CHEMISTRY,89(7),4071-4076.
MLA	Zhai, Guijin,et al."DNA-Templated Aptamer Probe for Identification of Target Proteins".ANALYTICAL CHEMISTRY 89.7(2017):4071-4076.