Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans

doi:10.1021/acsami.8b02423

	Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans
	Li, Xin 1,2; Liu, Shengju 1,2; Zhang, Hongyan 1,2; Mao, Jiawei 1,2; Niu, Huan 1,2; Lv, Wenping 1; Zhao, Xingyun 1,2; Wu, Ren'an 1; Xu, Guiju 1,2; Peng, Jiaxi 1,2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018-04-11
卷号	10 期号:14 页码:11896-11906
关键词	Metal-free Porous Graphitic Carbon Metal-organic Framework Glycan Profiling Enrichment Size Exclusion
ISSN号	1944-8244
DOI	10.1021/acsami.8b02423
文献子类	Article
英文摘要	In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material. The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m(2)/g. After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism. Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 mu g/mu L, and the detection limit of glycans could be down to 1 ng/mu L, with 21 N-linked glycans identified. When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified. From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon. In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
WOS关键词	OXYGEN REDUCTION REACTION ; MALDI-TOF-MS ; MASS-SPECTROMETRY ; SELECTIVE ENRICHMENT ; LASER DESORPTION/IONIZATION ; LIQUID-CHROMATOGRAPHY ; GRAPHENE NANORIBBONS ; NANOPOROUS CARBONS ; FACILE PREPARATION ; HIGH-THROUGHPUT
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000430156000058
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169078]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wu, Ren'an
作者单位	1.Chinese Acad Sci, Lab High Resolut Mass Spectrometry Technol, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Xin,Liu, Shengju,Zhang, Hongyan,et al. Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(14):11896-11906.
APA	Li, Xin.,Liu, Shengju.,Zhang, Hongyan.,Mao, Jiawei.,Niu, Huan.,...&Peng, Jiaxi.(2018).Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans.ACS APPLIED MATERIALS & INTERFACES,10(14),11896-11906.
MLA	Li, Xin,et al."Highly Porous Metal-Free Graphitic Carbon Derived from Metal-Organic Framework for Profiling of N-Linked Glycans".ACS APPLIED MATERIALS & INTERFACES 10.14(2018):11896-11906.