A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems

doi:10.1016/j.talanta.2018.01.076

	A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems
	Feng, Chunbo 2; Guan, Yafeng 2; Ning, Haijing 2; Geng, Xuhui 2; Shi, Meng 1,2
刊名	TALANTA
	2018-05-15
卷号	182 页码:279-284
关键词	Laser Induced Fluorescence Compact Low-cost Single Cell
ISSN号	0039-9140
DOI	10.1016/j.talanta.2018.01.076
文献子类	Article
英文摘要	A compact and low-cost laser induced fluorescence (LIF) detector based on confocal structure for capillary flow systems was developed and applied for analysis of Her2 protein on single Hela cells. A low-power and low-cost 450 nm laser diode (LD) instead of a high quality laser was used as excitation light source. A compact optical design together with shortened optical path length improved the optical efficiency and detection sensitivity. A superior silicon based photodetector assembly was used for fluorescence detection instead of a photomultiplier (PMT). The limit of detection (LOD) for fluorescein sodium was 3 x 10(-12) M or 165 fluorescein molecules in detection volume measured on a homemade capillary electroosmotic driven (EOD)-LIF system, which was similar to commercial LIFs. Compared to commercial LIFs, the whole volume of our LIF was reduced to 1/2-1/3, and the cost was less than 1/3 of them.
WOS关键词	LIGHT-EMITTING-DIODES ; SINGLE-CELL ; LIQUID-CHROMATOGRAPHY ; ELECTROPHORESIS
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000428229200035
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/169014]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Guan, Yafeng
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Feng, Chunbo,Guan, Yafeng,Ning, Haijing,et al. A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems[J]. TALANTA,2018,182:279-284.
APA	Feng, Chunbo,Guan, Yafeng,Ning, Haijing,Geng, Xuhui,&Shi, Meng.(2018).A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems.TALANTA,182,279-284.
MLA	Feng, Chunbo,et al."A compact and low-cost laser induced fluorescence detector with silicon based photodetector assembly for capillary flow systems".TALANTA 182(2018):279-284.