Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell

doi:10.1016/j.ijbiomac.2018.01.113

	Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell
	Du, Yuguang 3; Liu, Dongdong 1; Jiao, Siming 3; Cheng, Gong 3; Li, Xueming 1; Pei, Zhichao 1; Pei, Yuxin 1; Yin, Heng 2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-05-01
卷号	111 页码:1083-1090
关键词	Chitosan Oligosaccharides (Cos) Cos Binding Proteins Plasma Membrane Wheat Leaf Cell Plant Immune System
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2018.01.113
文献子类	Article
英文摘要	Chitosan oligosaccharides (COS) have the ability to improve plant resistance to pests and diseases through activating plant immune system. However, it remains unclear whether stimulating reason of COS was associated with the plasma membrane proteins. Here, the interaction of COS with wheat leaf cell demonstrated that fluorescence-labeled COS were enriched on the cell surface and the interaction of COS with plasma membrane proteins was confirmed by quartz crystal microbalance (QCM) biosensor. What's more, HPLC and SDS-PAGE analysis showed that COS binding proteins exhibited more than three peaks and the molecular weight were 66 kDa to 97 kDa, where the COS binding proteins were fished out from wheat plasma membrane proteins by the COS affinity column. More importantly, LC-MS/MS analysis demonstrated that several candidates, including W5G2U8_WHEAT (a potential wall-associated receptor kinase protein), W5HY42_WHEAT and W510R4_WHEAT (potential G-type lectin S-receptor-like serine/threonine-protein kinase), have the potential to be COS receptors. (C) 2018 Elsevier B.V. All rights reserved.
WOS关键词	WALL-ASSOCIATED KINASES ; TOBACCO-MOSAIC-VIRUS ; PATTERN-RECOGNITION RECEPTORS ; CHITIN ELICITOR ; DEFENSE PROTEINS ; INNATE IMMUNITY ; PLANT-LECTINS ; H+-ATPASE ; ARABIDOPSIS ; RESISTANCE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000429391000122
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168996]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Du, Yuguang; Pei, Yuxin; Yin, Heng
作者单位	1.Northwest A&F Univ, Coll Chem & Pharm, Shaanxi Key Lab Nat Prod & Chem Biol, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycoconjugate Res Grp, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Natl Engn Res Ctr Biotechnol Beijing,Inst Proc En, Army Key Lab Biopharmaceut Mfg & Dosage Form Engn, 1 North 2nd St, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Du, Yuguang,Liu, Dongdong,Jiao, Siming,et al. Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,111:1083-1090.
APA	Du, Yuguang.,Liu, Dongdong.,Jiao, Siming.,Cheng, Gong.,Li, Xueming.,...&Yin, Heng.(2018).Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,111,1083-1090.
MLA	Du, Yuguang,et al."Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 111(2018):1083-1090.