Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography

doi:10.1016/j.chroma.2018.01.028

	Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography
	Ma, Shujuan 2,3; Zhang, Haiyang 2,3; Li, Ya 2,3; Li, Yanan 1,3; Zhang, Na 1,3; Ou, Junjie 3; Ye, Mingliang 3; Wei, Yinmao 2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2018-02-23
卷号	1538 页码:8-16
关键词	Hybrid Monoliths Thiol-yne Click Reaction Photo-initiated Polymerization Capillary Liquid Chromatography 1 1 3-diethynyltetramethyl-disiloxane 6-hexanedithiol
ISSN号	0021-9673
DOI	10.1016/j.chroma.2018.01.028
文献子类	Article
英文摘要	Although several approaches have been developed to fabricate hybrid monoliths, it would still take a few hours to finish the formation of monoliths. Herein, photo-initiated thiol-yne polymerization was first adopted to in situ fabricate hybrid monoliths within the confines of UV-transparent fused-silica capillary. A silicon-containing diyne (1,3-diethynyltetramethyl-disiloxane, DYDS) was copolymerized with three multithiols, 1,6-hexanedithiol, trimethylolpropane tris(3-mercaptopropionate) and pentaerythriol tetrakis(3-mercaptopropionate), by using a binary porogenic system of diethylene glycol diethyl ether (DEGDE)/poly(ethylene glycol) (PEG200) within 10 min. Several characterizations of three hybrid monoliths (assigned as I, II and III, respectively) were performed. The results showed that these hybrid monoliths possessed bicontinuous porous structure, which was remarkably different from that via typical free-radical polymerization. The highest column efficiency of 76,000 plates per meter for butylbenzene was obtained on the column I in reversed-phase liquid chromatography (RPLC). It was observed that the efficiencies for strong-retained butylbenzene were almost close to those of weak-retained benzene, indicating a retention-independent efficient performance of small molecules on hybrid column I. The surface area of this hybrid monolith was very small in the dry state (less than 10.0 m(2)/g), and the chromatographic behavior of hybrid monolithic columns would be possibly explained by radical-mediated step-growth process of thiol-yne polymerization. Finally, the column I was applied for separation of BSA tryptic digest by cLC-MS/MS, indicating satisfactory separation ability for complicated samples. (C) 2018 Elsevier B.V. All rights reserved.
WOS关键词	ENE CLICK CHEMISTRY ; SMALL MOLECULES ; ACRYLATE POLYMERIZATION ; FACILE PREPARATION ; PERFORMANCE ; SEPARATION ; EFFICIENCY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000426230700002
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168886]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wei, Yinmao
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Northwest Univ, Minist Educ, Coll Chem & Mat Sci, Key Lab Synth & Nat Funct Mol Chem, Xian 710069, Shaanxi, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ma, Shujuan,Zhang, Haiyang,Li, Ya,et al. Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1538:8-16.
APA	Ma, Shujuan.,Zhang, Haiyang.,Li, Ya.,Li, Yanan.,Zhang, Na.,...&Wei, Yinmao.(2018).Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1538,8-16.
MLA	Ma, Shujuan,et al."Fast preparation of hybrid monolithic columns via photo-initiated thiol-yne polymerization for capillary liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1538(2018):8-16.