Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry

doi:10.1016/j.chroma.2017.09.061

	Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry
	Zhang, Haiyang 1,2; Ma, Shujuan 1,2; Yao, Yating 2; Li, Yanan 2; Li, Ya 1,2; Ou, Junjie 2; Ye, Mingliang 2; Wei, Yinmao 1
刊名	JOURNAL OF CHROMATOGRAPHY A
	2017-11-17
卷号	1524 页码:135-142
关键词	Hybrid Monolith Polyhedral Oligomertic Silsesquioxane Click Reaction Two-dimensional Separation
ISSN号	0021-9673
DOI	10.1016/j.chroma.2017.09.061
文献子类	Article
英文摘要	A facile approach was developed to prepare hybrid monoliths with different functions via two-step photo-initiated reactions. Firstly, acrylopropyl polyhedral oligomertic silsesquioxane (acryl-POSS) and propargyl acrylate(PA) were used as precursors to synthesize alkynyl-functionalized hybrid monoliths via photo-initiated free radical polymerization. Secondly, the hybrid monoliths were modified with 1-octadecanethiol (ODT) and sodium 3-mercapto-l-propanesulfonate (SMPS) via photo-initiated thiolyne click reaction to prepare reversed-phase (RP) and strong cation-exchange (SCX) hybrid monoliths, respectively. The results of chromatographic characterization indicated that the column efficiencies for alkylbenzenes on ODT-modified hybrid monolith reached 84,000-87,700 plates per meter at the velocity of 0.58 mm/s, and also revealed a retention-independent efficient performance of small molecules in isocratic elution. The SMPS-modified hybrid monolith exhibited both hydrophobicity and ion-exchange mechanisms, and the dynamic binding capacity was calculated to be 1.4 x 10(-4) mu mol/cm. Human Hela cells tryptic digest was well separated on ODT-modified hybrid monolith in one-dimensional RPLC-MS/MS, and 2786 unique peptides and 685 proteins were identified. Furthermore, the SMPSmodified monolith coupled with ODT-modified monolith was used for two-dimensional separation of human Hela cells tryptic digest in SCX-RPLC-MS/MS, and the results showed that 9744 unique peptides and 2749 proteins were identified. Compared to those identified in one-dimensional RP system, the total numbers of unique peptides and proteins identified in SCX-RP system increased by 249.7% and 301.3%, respectively. (C) 2017 Elsevier B.V. All rights reserved.
WOS关键词	ENE CLICK CHEMISTRY ; POLYHEDRAL OLIGOMERIC SILSESQUIOXANE ; SHOTGUN PROTEOME ANALYSIS ; POROUS POLYMER MONOLITHS ; METAL-ORGANIC FRAMEWORK ; CAPILLARY COLUMN ; SMALL MOLECULES ; TRAP COLUMN ; PERFORMANCE ; SEPARATION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000415772100014
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/168330]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wei, Yinmao
作者单位	1.Northwest Univ, Coll Chem & Mat Sci, Minist Educ, Key Lab Synthet & Nat Funct Mol Chem, Xian 710127, Shaanxi, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Haiyang,Ma, Shujuan,Yao, Yating,et al. Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1524:135-142.
APA	Zhang, Haiyang.,Ma, Shujuan.,Yao, Yating.,Li, Yanan.,Li, Ya.,...&Wei, Yinmao.(2017).Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1524,135-142.
MLA	Zhang, Haiyang,et al."Facile preparation of multi-functionalized hybrid monoliths via two-step photo-initiated reactions for two-dimensional liquid chromatography-mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1524(2017):135-142.