Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy

doi:10.1021/ja508484c

	Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy
	Rong, Jie 1,2; Han, Jing 3; Dong, Lu 2,5; Tan, Yanhong 2; Yang, Huaqian 3; Feng, Lianshun 1,2; Wang, Qi-Wei 3; Meng, Rong 2,5; Zhao, Jing 1,6; Wang, Shi-Qiang 3
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2014-12-17
卷号	136 期号:50 页码:17468-17476
ISSN号	0002-7863
DOI	10.1021/ja508484c
文献子类	Article
英文摘要	In the heart, glycosylation is involved in a variety of physiological and pathological processes. Cardiac glycosylation is dynamically regulated, which remains challenging to monitor in vivo. Here we describe a chemical approach for analyzing the dynamic cardiac glycome by metabolically labeling the cardiac glycans with azidosugars in living rats. The azides, serving as a chemical reporter, are chemoselectively conjugated with fluorophores using copper-free click chemistry for glycan imaging; derivatizing azides with affinity tags allows enrichment and proteomic identification of glycosylated cardiac proteins. We demonstrated this methodology by visualization of the cardiac sialylated glycans in intact hearts and identification of more than 200 cardiac proteins modified with sialic acids. We further applied this methodology to investigate the sialylation in hypertrophic hearts. The imaging results revealed an increase of sialic acid biosynthesis upon the induction of cardiac hypertrophy. Quantitative proteomic analysis identified multiple sialylated proteins including neural cell adhesion molecule 1, T-kininogens, and alpha(2)-macroglobulin that were upregulated during hypertrophy. The methodology may be further extended to other types of glycosylation, as exemplified by the mucin-type O-linked glycosylation. Our results highlight the applications of metabolic glycan labeling coupled with bioorthogonal chemistry in probing the biosynthesis and function of cardiac glycome during pathophysiological responses.
WOS关键词	O-LINKED GLYCOSYLATION ; FREE CLICK CHEMISTRY ; LIVING ANIMALS ; COPPER-FREE ; CARDIOMYOCYTE HYPERTROPHY ; VENTRICULAR MYOCYTES ; MASS-SPECTROMETRY ; MODIFIED PROTEINS ; FAILING HEART ; CELL-SURFACES
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000346682600025
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/167302]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Zhao, Jing
作者单位	1.Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Shenzhen 518055, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China 3.Peking Univ, State Key Lab Biomembrane & Membrane Biotechnol, Coll Life Sci, Beijing 100871, Peoples R China 4.Peking Univ, Synthet & Funct Biomol Ctr, Beijing 100871, Peoples R China 5.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China 6.Nanjing Univ, Inst Chem & Biomed Sci, State Key Lab Pharmaceut Biotechnol, Sch Life Sci, Nanjing 210093, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Rong, Jie,Han, Jing,Dong, Lu,et al. Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2014,136(50):17468-17476.
APA	Rong, Jie.,Han, Jing.,Dong, Lu.,Tan, Yanhong.,Yang, Huaqian.,...&Chen, Xing.(2014).Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,136(50),17468-17476.
MLA	Rong, Jie,et al."Glycan Imaging in Intact Rat Hearts and Glycoproteomic Analysis Reveal the Upregulation of Sialylation during Cardiac Hypertrophy".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 136.50(2014):17468-17476.