Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid

doi:10.1002/elps.201200122

	Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid
	Chang, Cuilan; Liu, Huwei; Zhou, Zhigui; Zhao, Meiping; Yang, Youyou; Han, Yehua; Bai, Yu
刊名	ELECTROPHORESIS
	2012-11-01
卷号	33 期号:22 页码:3387-3393
关键词	Chiral Separation Dart-ms Jasmonic Acid 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol Nplc
ISSN号	0173-0835
DOI	10.1002/elps.201200122
文献子类	Article
英文摘要	Normal phase chiral LC (NPLC) has been proved to be powerful and efficient for chiral separation. However, the combination of NPLC with ESI or atmospheric pressure chemical ionization MS is restricted by the poor ionization efficiency and thermal fragmentations of analytes to some extent. Direct analysis in real time MS (DART-MS) is an ambient ionization technique that shows high ionization efficiency of the analytes in the normal phase mobile phase. In this work, we coupled chiral NPLC to DART-MS for the chiral qualitative and quantitative analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid enantiomers. Satisfactory results for the enantiomers of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol operating in the positive mode were obtained in terms of linearity (2.5250 mu g/mL, R-2, 0.999-1.000) and repeatability (25 mu g/mL, RSDs, 4.7-5.6%). Moreover, chiral NPLC-DART-MS resulted in the simultaneous chiral separation and detection of jasmonic acid enantiomers, which are very difficult to be analyzed by NPLC-ESI-MS and NPLC-APCI-MS. Compared with the coupled techniques of NPLC-ESI-MS and NPLC-APCI-MS, NPLC-DART-MS showed advantages in increasing the ionization efficiency and reducing the in-source thermal fragmentation of analytes.
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; FLIGHT MASS-SPECTROMETRY ; ATMOSPHERIC-PRESSURE PHOTOIONIZATION ; DART-MS ; ELECTROSPRAY-IONIZATION ; HUMAN PLASMA ; ION-SOURCE ; S-OXIDE ; RESOLUTION ; PHARMACEUTICALS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000311303100018
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/167274]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Liu, Huwei
作者单位	Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn,Inst Analyt Chem,Minist Educ, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Chang, Cuilan,Liu, Huwei,Zhou, Zhigui,et al. Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid[J]. ELECTROPHORESIS,2012,33(22):3387-3393.
APA	Chang, Cuilan.,Liu, Huwei.,Zhou, Zhigui.,Zhao, Meiping.,Yang, Youyou.,...&Bai, Yu.(2012).Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid.ELECTROPHORESIS,33(22),3387-3393.
MLA	Chang, Cuilan,et al."Normal phase LC coupled with direct analysis in real time MS for the chiral analysis of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol and jasmonic acid".ELECTROPHORESIS 33.22(2012):3387-3393.