Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm

doi:10.1016/j.molcel.2018.08.011

	Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm
	Shi, Hailing 1,6,7; Wei, Jiangbo 1,6,7; Liu, Fange 1,6,7; Lu, Zhike 1,2,6,7; Fei, Qili 1,6,7; Ai, Yuxi 1,6,7; He, P. Cody 1,6,7; Cui, Xiaolong 1,6,7; Su, Rui 3; Klungland, Arne 5
刊名	MOLECULAR CELL
	2018-09-20
卷号	71 期号:6 页码:973-+
ISSN号	1097-2765
DOI	10.1016/j.molcel.2018.08.011
通讯作者	He, Chuan(chuanhe@uchicago.edu)
英文摘要	FTO, the first RNA demethylase discovered, mediates the demethylation of internal N-6-methyladenosine (m(6)A) and N-6, 2-O-dimethyladenosine (m(6)A(m)) at the +1 position from the 5' cap in mRNA. Here we demonstrate that the cellular distribution of FTO is distinct among different cell lines, affecting the access of FTO to different RNA substrates. We find that FTO binds multiple RNA species, including mRNA, snRNA, and tRNA, and can demethylate internal m(6)A and cap m(6)A(m) in mRNA, internal m(6)A in U6 RNA, internal and cap m(6)A(m) in snRNAs, and N-1-methyladenosine (m(1)A) in tRNA. FTO-mediated demethylation has a greater effect on the transcript levels of mRNAs possessing internal m(6)A than the ones with cap m(6)A(m) in the tested cells. We also show that FTO can directly repress translation by catalyzing m(1)A tRNA demethylation. Collectively, FTO-mediated RNA demethylation occurs to m(6)A and m(6)A(m) in mRNA and snRNA as well as m(1)A in tRNA.
资助项目	U.S. National Insititutes of Health[GM071440] ; U.S. National Insititutes of Health[HG008935] ; U.S. National Insititutes of Health[CA214965] ; National Basic Research Program of China[2014CB964900] ; U.S. National Science Foundation[CHE-1048528] ; U.S. National Institutes of Health[CA014599]
WOS关键词	OBESITY-ASSOCIATED FTO ; PRE-MESSENGER-RNA ; SUBSTRATE-SPECIFICITY ; CRYSTAL-STRUCTURES ; METHYLATION ; GENE ; N6-METHYLADENOSINE ; TRANSLATION ; REVEALS ; ALKB
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000445103900010
资助机构	U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health ; U.S. National Insititutes of Health ; U.S. National Insititutes of Health ; National Basic Research Program of China ; National Basic Research Program of China ; U.S. National Science Foundation ; U.S. National Science Foundation ; U.S. National Institutes of Health ; U.S. National Institutes of Health
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/167040]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	He, Chuan
作者单位	1.Univ Chicago, Inst Biophys Dynam, 929 East 57 St, Chicago, IL 60637 USA 2.Westlake Univ, Westlake Inst Adv Study, Inst Nat Sci, 18 Shilongshan Rd, Hangzhou 310064, Zhejiang, Peoples R China 3.Beckman Res Inst City Hope, Dept Syst Biol, Monrovia, CA 91016 USA 4.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing Natl Lab Mol Sci,Minist Educ,Coll Chem &, Beijing 100871, Peoples R China 5.Univ Oslo, Norway Inst Basic Med Sci, Inst Med Microbiol, Oslo Univ Hosp,Rikshosp, POB 1018 Blindern, N-0315 Oslo, Norway 6.Univ Chicago, Dept Biochem & Mol Biol, Dept Chem, 929 East 57 St, Chicago, IL 60637 USA 7.Univ Chicago, Howard Hughes Med Inst, 929 East 57 St, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Shi, Hailing,Wei, Jiangbo,Liu, Fange,et al. Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm[J]. MOLECULAR CELL,2018,71(6):973-+.
APA	Shi, Hailing.,Wei, Jiangbo.,Liu, Fange.,Lu, Zhike.,Fei, Qili.,...&He, Chuan.(2018).Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm.MOLECULAR CELL,71(6),973-+.
MLA	Shi, Hailing,et al."Differential m(6)A, m(6)A(m), and m(1)A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm".MOLECULAR CELL 71.6(2018):973-+.