Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

	Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere
	Qin, Zhi Qiang 1; Xiao, Pei Gen 4; An, Bai Lin 1; Hao, Da Cheng 1; Song, Si Meng 1; Cheng, Yan 1; Ge, Guang Bo 2,3
刊名	POLISH JOURNAL OF MICROBIOLOGY
	2018
卷号	67 期号:4 页码:417-429
关键词	Myrothecium reactive dye decolorization laccase transcriptome sequencing degradation mechanism
ISSN号	1733-1331
通讯作者	Hao, Da Cheng(hao@djtu.edu.cn)
英文摘要	We isolated three laccase-producing fungus strains from Taxus rhizosphere. Myrotheium verrucaria strain DJTU-sh7 had the highest laccase activity of 216.2 U/ml, which was increased to above 300 U/ml after optimization. DJTU-sh7 had the best decolorizing effect for three classes of reactive dyes. The DJTU-sh7-containing fungal consortium displayed the robust decolorizing ability. Both color removal efficiency and chemical oxygen demand were increased in the consortium mediated biotransformation. Transcriptome changes of M. verrucaria elicited by azo dye and phenolic were quantified by the high throughput transcriptome sequencing, and the activities of the selected oxidases and reductases were determined. The possible involvement of oxidases and reductases, especially laccase, aryl alcohol oxidase, and ferric reductase in the biotransformation of dye and phenolic compounds was revealed at both transcriptomic and phenotypic levels. Revealing the transcriptomic mechanisms of fungi in dealing with organic pollutants facilitates the fine-tuned manipulation of strains in developing novel bioremediation and biodegradation strategies.
资助项目	Liaoning Postgraduate Education and Teaching Reform Project ; Natural Science Fund of Liaoning province[2015020663] ; Natural Science Fund of Liaoning province[20180550190]
WOS关键词	MALACHITE GREEN DECOLORIZATION ; AZO DYES ; DEGRADATION ; OXIDASE ; ENZYMES ; BIODEGRADATION ; PREDICTION ; REDUCTION ; TOLERANCE ; MECHANISM
WOS研究方向	Microbiology
语种	英语
出版者	POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS
WOS记录号	WOS:000455563500003
资助机构	Liaoning Postgraduate Education and Teaching Reform Project ; Liaoning Postgraduate Education and Teaching Reform Project ; Natural Science Fund of Liaoning province ; Natural Science Fund of Liaoning province ; Liaoning Postgraduate Education and Teaching Reform Project ; Liaoning Postgraduate Education and Teaching Reform Project ; Natural Science Fund of Liaoning province ; Natural Science Fund of Liaoning province ; Liaoning Postgraduate Education and Teaching Reform Project ; Liaoning Postgraduate Education and Teaching Reform Project ; Natural Science Fund of Liaoning province ; Natural Science Fund of Liaoning province ; Liaoning Postgraduate Education and Teaching Reform Project ; Liaoning Postgraduate Education and Teaching Reform Project ; Natural Science Fund of Liaoning province ; Natural Science Fund of Liaoning province
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166234]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Hao, Da Cheng
作者单位	1.Dalian Jiaotong Univ, Sch Environm & Chem Engn, Biotechnol Inst, Dalian, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 3.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China 4.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Qin, Zhi Qiang,Xiao, Pei Gen,An, Bai Lin,et al. Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere[J]. POLISH JOURNAL OF MICROBIOLOGY,2018,67(4):417-429.
APA	Qin, Zhi Qiang.,Xiao, Pei Gen.,An, Bai Lin.,Hao, Da Cheng.,Song, Si Meng.,...&Ge, Guang Bo.(2018).Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere.POLISH JOURNAL OF MICROBIOLOGY,67(4),417-429.
MLA	Qin, Zhi Qiang,et al."Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere".POLISH JOURNAL OF MICROBIOLOGY 67.4(2018):417-429.