Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization

doi:10.1021/acs.jafc.8b04667

	Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization
	Ji, Chaofan 1; Liang, Huipeng 1; Li, Shengjie 1; Yu, Chenxu 3; Lin, Xinping 1; Wang, Zhenyu 1; Bao, Ruiqi 1; Gao, Ning 2; Lv, Jing 1
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2019-01-30
卷号	67 期号:4 页码:1156-1164
关键词	Rhodosporidium toruloides torularhodin Agrobacterium tumefaciens-mediated transformation response surface methodology selective production
ISSN号	0021-8561
DOI	10.1021/acs.jafc.8b04667
通讯作者	Wang, Zhenyu(wangzy0506@outlook.com) ; Lin, Xinping(yingchaer@163.com)
英文摘要	Nine transformants of Rhodosporidium toruloides with significant changes in the carotenoid profile were obtained by Agrobacterium tumefaciens-mediated transformation, including a white, three red, and four yellow mutants. A red mutant Al-15-BRQ that showed a high torularhodin production was selected for culture condition optimization. Results indicated that the torularhodin yield was boosted with glucose as the carbon source, at a carbon/nitrogen ratio of 22, a loading volume of 75 mL, and 28 degrees C. The torularhodin yield of 21.3 mg/L consisting of 94.4% total carotenoids was obtained by Box-Behnken design experiments. The torularhodin yield was 17.0-fold higher than that of the wild type, with time shortened from 9 to 3 days. This study reports an effective strategy for improving torularhodin production and provides a candidate R toruloides strain for highly selective production of torularhodin.
资助项目	National Natural Science Foundation of China[31501464] ; High Level Talents Innovation and Entrepreneurship Project of Dalian[2017RQ045] ; Natural Science Foundation of Liaoning Province[20170540057] ; Educational Commission of Liaoning Province[2016J018]
WOS关键词	YEAST RHODOTORULA-GLUTINIS ; CAROTENOID PRODUCTION ; BETA-CAROTENE ; LIPID PRODUCTION ; C/N RATIO ; GLYCEROL ; GROWTH ; MUTANT ; TEMPERATURE ; PROFILES
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000457815800014
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; High Level Talents Innovation and Entrepreneurship Project of Dalian ; Natural Science Foundation of Liaoning Province ; Natural Science Foundation of Liaoning Province ; Educational Commission of Liaoning Province ; Educational Commission of Liaoning Province
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/166150]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Lin, Xinping; Wang, Zhenyu
作者单位	1.Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, 1 Qinggongyuan, Dalian 116034, Liaoning, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 3.Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA
推荐引用方式 GB/T 7714	Ji, Chaofan,Liang, Huipeng,Li, Shengjie,et al. Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(4):1156-1164.
APA	Ji, Chaofan.,Liang, Huipeng.,Li, Shengjie.,Yu, Chenxu.,Lin, Xinping.,...&Lv, Jing.(2019).Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(4),1156-1164.
MLA	Ji, Chaofan,et al."Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.4(2019):1156-1164.