Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites

doi:10.1002/chem.201900539

	Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites
	Zeng, Jun 2,6; Wang, Zhichao 2,6; Huang, Xin 3; Eckstein, Sabine S.1,4,5; Lin, Xiaohui 3; Piao, Hailong 2; Weigert, Cora 1,4,5; Yin, Peiyuan 2; Lehmann, Rainer 1,4,5; Xu, Guowang 2
刊名	CHEMISTRY-A EUROPEAN JOURNAL
	2019-04-11
卷号	25 期号:21 页码:5427-5432
关键词	capillary electrophoresis isotopologues mass spectrometry metabolism metabolomics
ISSN号	0947-6539
DOI	10.1002/chem.201900539
通讯作者	Lehmann, Rainer(rainer.lehmann@med.uni-tuebingen.de) ; Xu, Guowang(xugw@dicp.ac.cn)
英文摘要	Mass spectrometry (MS) driven metabolomics is a frequently used tool in various areas of life sciences; however, the analysis of polar metabolites is less commonly included. In general, metabolomic analyses lead to the detection of the total amount of all covered metabolites. This is currently a major limitation with respect to metabolites showing high turnover rates, but no changes in their concentration. Such metabolites and pathways could be crucial metabolic nodes (e.g., potential drug targets in cancer metabolism). A stable-isotope tracing capillary electrophoresis-mass spectrometry (CE-MS) metabolomic approach was developed to cover both polar metabolites and isotopologues in a non-targeted way. An in-house developed software enables high throughput processing of complex multidimensional data. The practicability is demonstrated analyzing [U-C-13]-glucose exposed prostate cancer and non-cancer cells. This CE-MS-driven analytical strategy complements polar metabolite profiles through isotopologue labeling patterns, thereby improving not only the metabolomic coverage, but also the understanding of metabolism.
资助项目	National Key R&D Program of China[2017YFC0906900] ; National Natural Science Foundation of China[21707042] ; National Natural Science Foundation of China[81472374] ; National Natural Science Foundation of China[21375011] ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG)[GZ 753] ; Sino-German Center for Research Promotion (DFG)[LE 1391/1-1]
WOS关键词	HEPATOCELLULAR-CARCINOMA ; PROSTATE-CANCER ; SERINE ; BIOMARKERS
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000466018300009
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG) ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (NSFC) ; Sino-German Center for Research Promotion (DFG) ; Sino-German Center for Research Promotion (DFG)
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/165522]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Lehmann, Rainer; Xu, Guowang
作者单位	1.German Ctr Diabet Res DZD, Tubingen, Germany 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Dalian Univ Technol, Dept Comp Sci & Engn, Dalian 116024, Peoples R China 4.Univ Hosp Tubingen, Dept Diagnost Lab Med, Inst Clin Chem & Pathobiochem, D-72076 Tubingen, Germany 5.Univ Hosp Tubingen, Inst Diabet Res & Metab Dis, German Ctr Diabet Res DZD, Clin Chem Lab,Core Facil, D-72076 Tubingen, Germany 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Jun,Wang, Zhichao,Huang, Xin,et al. Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites[J]. CHEMISTRY-A EUROPEAN JOURNAL,2019,25(21):5427-5432.
APA	Zeng, Jun.,Wang, Zhichao.,Huang, Xin.,Eckstein, Sabine S..,Lin, Xiaohui.,...&Xu, Guowang.(2019).Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites.CHEMISTRY-A EUROPEAN JOURNAL,25(21),5427-5432.
MLA	Zeng, Jun,et al."Comprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry: A New Tool Complementing Metabolomic Analyses of Polar Metabolites".CHEMISTRY-A EUROPEAN JOURNAL 25.21(2019):5427-5432.