Selectively screen the antibacterial peptide from the hydrolysates of highland barley

	Selectively screen the antibacterial peptide from the hydrolysates of highland barley
	Pei, Jinjin ; Feng, Zhenzhen ; Ren, Ting ; Jin, Wengang ; Li, Xinsheng ; Chen, Dejing ; Tao, Yanduo ; Dang, Jun
刊名	ENGINEERING IN LIFE SCIENCES
	2018
英文摘要	Highland barley is one of the most important industrial crops in Tibetan plateau. Previous research indicated that highland barley has many medical functions. In this work, the antibacterial abilities of highland barley were investigated. The protein solutions hydrolyzed by trypsin for 4 h exhibited the highest antibacterial activity. An antibacterial peptide, barleycin, was screened and purified by magnetic liposome extraction combining with the protein profiles of reversed-phase high-performance liquid chromatography (RP-HPLC). Structure, characterization, and safety evaluation of barleycin were further investigated. Amino acids sequence was determined as Lys-Ile-Ile-Ile-Pro-Pro-Leu-Phe-His by N-sequencing. Circular dichroism spectra indicated the a-helix conformation of barleycin. The activity spectrum included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua and Escherichia coli and the MICs were from 4 to 16g/mL. Safety evaluations with cytotoxicity and hemolytic suggested this antibacterial peptide could be considered as safe at MICs. Finally, mode of action of barleycin on sensitive cells was primarily studied. The results suggested the damage of cell membrane.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/10382]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Pei, Jinjin,Feng, Zhenzhen,Ren, Ting,et al. Selectively screen the antibacterial peptide from the hydrolysates of highland barley[J]. ENGINEERING IN LIFE SCIENCES,2018.
APA	Pei, Jinjin.,Feng, Zhenzhen.,Ren, Ting.,Jin, Wengang.,Li, Xinsheng.,...&Dang, Jun.(2018).Selectively screen the antibacterial peptide from the hydrolysates of highland barley.ENGINEERING IN LIFE SCIENCES.
MLA	Pei, Jinjin,et al."Selectively screen the antibacterial peptide from the hydrolysates of highland barley".ENGINEERING IN LIFE SCIENCES (2018).