Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system

	Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system
	Yan, Xiaolu ; Zhao, Xian-En ; Sun, Jing ; Zhu, Shuyun ; Lei, Cuihua ; Li, Ru ; Gong, Peiwei ; Ling, Baoping ; Wang, Renjun ; Wang, Hua
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2018-06-15
英文摘要	Graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) were combined for probing glutathione reductase (GR) activity. The main probing mechanism is based on the following facts: (1) The fluorescence of GQDs can be quenched by AuNPs through fluorescence resonance energy transfer (FRET). (2) Reduced glutathione (GSH) can protect AuNPs from being aggregated and enlarge the inter-particle distance between GQDs and AuNPs, which subsequently produces the fluorescent signal recovery. (3) AuNPs has much weaker affinity towards oxidized glutathione (GSSG) in contrast to GSH. (4) GR can catalyze the reduction of GSSG into GSH in the presence of coenzyme beta-nicotinamide adenine dinucleotide 2'-phophate hydrate (NADPH). Based on these facts, a turn-on fluorescent assay for GR has thus been developed based on the GQDs-AuNPs system. The proposed assay can allow for the evaluation of GR activity in the range of 0.0050-0.13 mU/mL, with a minimum detectable concentration of 0.0050 mU/mL, which was more than one order of magnitude lower than most of the current sensing platforms. Besides, this GQDs-AuNPs based GR assay was successfully applied for screening the inhibitor of GR activity by using 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) as a model. (C) 2018 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13342]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yan, Xiaolu,Zhao, Xian-En,Sun, Jing,et al. Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018.
APA	Yan, Xiaolu.,Zhao, Xian-En.,Sun, Jing.,Zhu, Shuyun.,Lei, Cuihua.,...&Wang, Hua.(2018).Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system.SENSORS AND ACTUATORS B-CHEMICAL.
MLA	Yan, Xiaolu,et al."Probing glutathione reductase activity with graphene quantum dots and gold nanoparticles system".SENSORS AND ACTUATORS B-CHEMICAL (2018).