Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis
Suo, YR ; Wang, HL ; Li, YL ; You, JM ; Wang, HQ
刊名CHROMATOGRAPHIA ; Suo Yourui, Wang Honglun, Li Yulin, You Jinmao, Wang Haiqing.Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis.CHROMATOGRAPHIA,2004,60(9-10):589-595
2004-11-01
英文摘要A rapid capillary electrophoresis method for the separation of five natural pharmacologically active compounds from extracted Rhodiola, namely salidroside, tyrosol, rhodionin, gallic acid and ethyl gallate has been developed. The separation of five natural pharmacologically active compounds was carried out in a fused-silica capillary with 14 mM boric acid, 30 mM SDS and 2.5% acetonitrile, adjusted to pH 10.7 with NaOH. Applied potential was 21 kV. The temperature of the capillary was maintained at 25 degreesC by the instrument thermostating system, with the correlation coefficients of 0.9805-0.9989 for migration time, and relative standards of < 3.52% for peak areas. The established method is rapid and reproducible for the separation of five natural pharmacologically compounds from extracts of Rhodiola with satisfactory results.; A rapid capillary electrophoresis method for the separation of five natural pharmacologically active compounds from extracted Rhodiola, namely salidroside, tyrosol, rhodionin, gallic acid and ethyl gallate has been developed. The separation of five natural pharmacologically active compounds was carried out in a fused-silica capillary with 14 mM boric acid, 30 mM SDS and 2.5% acetonitrile, adjusted to pH 10.7 with NaOH. Applied potential was 21 kV. The temperature of the capillary was maintained at 25 degreesC by the instrument thermostating system, with the correlation coefficients of 0.9805-0.9989 for migration time, and relative standards of < 3.52% for peak areas. The established method is rapid and reproducible for the separation of five natural pharmacologically compounds from extracts of Rhodiola with satisfactory results.
内容类型期刊论文
源URL[http://ir.nwipb.ac.cn/handle/363003/1386]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
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GB/T 7714
Suo, YR,Wang, HL,Li, YL,et al. Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis[J]. CHROMATOGRAPHIA, Suo Yourui, Wang Honglun, Li Yulin, You Jinmao, Wang Haiqing.Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis.CHROMATOGRAPHIA,2004,60(9-10):589-595,2004.
APA Suo, YR,Wang, HL,Li, YL,You, JM,&Wang, HQ.(2004).Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis.CHROMATOGRAPHIA.
MLA Suo, YR,et al."Analysis of five pharmacologically active compounds from Rhodiola for natural product drug discovery with capillary electrophoresis".CHROMATOGRAPHIA (2004).
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