Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography | |
Li, Hongmei ; Chen, Tao ; Sun, Jing ; Wang, Weiqing ; Li, Yulin | |
刊名 | JOURNAL OF SEPARATION SCIENCE |
2017-06-01 | |
英文摘要 | In our present study, two groups of xanthones isomers (1-hydroxy-3,5,8-trimethoxyxanthone and 1-hydroxy-3,7,8-trimethoxyxanthone; 1,8-dihydroxy-3,7-dimethoxyxanthone and 1,8-dihydroxy-3,5-dimethanolxanthone) and other two xanthones (3-methoxy-1,5,8-trihydroxyxanthone and 3,5-dimethoxy-1-hydroxyxanthone) were separated from Swertia franchetiana. First, a solvent system composed of petroleum ether/methanol/water (2:1:0.6, v/v) was developed for the liquid-liquid extraction of these xanthones from the crude extract. Then, an efficient method was established for the one-step separation of these six xanthones by high-speed countercurrent chromatography using n-hexane/ethyl acetate/methanol/ethanol/water (HEMEW; 6:4:4:2:4, v/v) as the solvent system. The results showed that liquid-liquid extraction could be well developed for efficient enrichment of target compounds. Additionally, high-speed countercurrent chromatography could be a powerful technology for separation xanthones isomers. It was found ethanol could be a good methanol substitute when the HEMEW system could not provide good separation factors. |
内容类型 | 期刊论文 |
源URL | [http://ir.nwipb.ac.cn/handle/363003/7085] |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Li, Hongmei,Chen, Tao,Sun, Jing,et al. Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017. |
APA | Li, Hongmei,Chen, Tao,Sun, Jing,Wang, Weiqing,&Li, Yulin.(2017).Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography.JOURNAL OF SEPARATION SCIENCE. |
MLA | Li, Hongmei,et al."Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography".JOURNAL OF SEPARATION SCIENCE (2017). |
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