Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification

	Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification
	Zhao, X ; Li, YL ; Suo, YR ; Shi, YW ; Chen, XM ; Zhang, HF ; Sun, XJ ; You, JM
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2006-02-01
英文摘要	A simple and sensitive method for the determination of free fatty acids using acridone-9-ethyl-p-toluenesulfonate (AETS) as fluorescence derivatization reagent by reversed-phase high-performance liquid chromatography has been developed. Studies on derivatization conditions indicate that free fatty acids react rapidly and smoothly with AETS in the presence of K2CO3 catalyst at 85 degrees C in N,N-dimethylformamide solvent to give the corresponding sensitively fluorescent derivatives with maximal yields close to 100% with a 6-fold molar reagent excess. Free fatty acid derivatives were separated on Eclipse XDB-C-8 column with a good baseline resolution. The identification of 19 fatty acid derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (APCI) source under positive-ion mode detection. The contents of 19 free fatty acids in soil and three kinds of bryophytes (tree moss, entodon macropodus, dicranum caesium) were determined. The results indicate that the bryophyte plants enrich an abundance of free fatty acids from soil. The fluorescence excitation and emission wavelengths of derivatives were set at lambda(ex)=404 and lambda(em)=440 nm, respectively. Most linear correlation coefficients for fatty acid derivatives are over 0.9996, and detection limits (at signal-to-noise of 3:1) are 12.28 similar to 43.69 fmol.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6858]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, X,Li, YL,Suo, YR,et al. Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006.
APA	Zhao, X.,Li, YL.,Suo, YR.,Shi, YW.,Chen, XM.,...&You, JM.(2006).Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Zhao, X,et al."Determination of free fatty acids from soil and bryophyte by high performance liquid chromatography with fluorescence detection and their mass spectrum identification".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2006).