Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique

	Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique
	Pei, Jinjin ; Jiang, Lei
刊名	INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
	2017-07-01
英文摘要	Andrias davidianus, the Chinese giant salamander, has been used in traditional Chinese medicine for many decades. However, no antimicrobial peptides (AMPs) have been described from A. davidianus until now. Here we describe a novel AMP (andricin 01) isolated from the mucus of A. davidianus. The peptide was recovered using an innovative magnetic cell membrane separation technique and was characterised using mass spectrometry and circular dichroism (CD) spectroscopy. Andricin 01 is comprised of ten amino acid residues with a total molecular mass of 955.1 Da. CD spectrum analysis gave results similar to the archetypal random coil spectrum, consistent with the three-dimensional rendering calculated by current bioinformatics tools. Andricin 01 was found to be inhibitory both to Gram-negative and Gram-positive bacteria. Furthermore, the peptide at the minimal bacterial concentration did not show cell cytotoxicity against human hepatocytes or renal cells and did not show haemolytic activity against red blood cells, indicating that is potentially safe and effective for human use. Andricin 01 shows promise as a novel antibacterial that may provide an insight into the development of new drugs. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/7057]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Pei, Jinjin,Jiang, Lei. Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique[J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,2017.
APA	Pei, Jinjin,&Jiang, Lei.(2017).Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique.INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS.
MLA	Pei, Jinjin,et al."Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique".INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS (2017).