Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography

	Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography
	Zou, Deng-lang ; Chen, Tao ; Li, Hong-mei ; Chen, Chen ; Zhao, Jing-yang ; Li, Yu-lin
刊名	JOURNAL OF SEPARATION SCIENCE
	2016-04-01
英文摘要	This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography. The ultrasound-assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high-speed counter-current chromatography without any pretreatment using n-hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by H-1 NMR spectroscopy.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/5724]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zou, Deng-lang,Chen, Tao,Li, Hong-mei,et al. Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2016.
APA	Zou, Deng-lang,Chen, Tao,Li, Hong-mei,Chen, Chen,Zhao, Jing-yang,&Li, Yu-lin.(2016).Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE.
MLA	Zou, Deng-lang,et al."Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE (2016).