Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)

	Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)
	Zhang, Qian ; Yang, Yan-Zhuo ; Wu, Gui-Li ; Zhang, Dong-Yuan ; Liu, Jian-Quan
刊名	CONSERVATION GENETICS ; Zhang Qian, Yang YanZhuo,Wu GuiLi, Zhang DongYuan,Liu JianQuan.Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae).CONSERVATION GENETICS,2008,9(3):767-769
	2008-06-01
英文摘要	Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined. In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species. In addition, all markers have been crossly checked in the other four congeneric species.; Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined. In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species. In addition, all markers have been crossly checked in the other four congeneric species.
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/1221]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Qian,Yang, Yan-Zhuo,Wu, Gui-Li,et al. Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)[J]. CONSERVATION GENETICS, Zhang Qian, Yang YanZhuo,Wu GuiLi, Zhang DongYuan,Liu JianQuan.Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae).CONSERVATION GENETICS,2008,9(3):767-769,2008.
APA	Zhang, Qian,Yang, Yan-Zhuo,Wu, Gui-Li,Zhang, Dong-Yuan,&Liu, Jian-Quan.(2008).Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae).CONSERVATION GENETICS.
MLA	Zhang, Qian,et al."Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)".CONSERVATION GENETICS (2008).