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	Domain a' of protein disulfide isomerase plays key role in inhibiting alpha-synuclein fibril formation
	Cheng, Han1,2; Wang, Lei1; Wang, Chih-chen1
刊名	Cell stress & chaperones
	2010-07-01
卷号	15期号:4页码:415-421
关键词	Protein disulfide isomerase Alpha-synuclein Fibril Isothermal titration calorimetry
ISSN号	1355-8145
DOI	10.1007/s12192-009-0157-2
通讯作者	Wang, chih-chen(chihwang@sun5.ibp.ac.cn)
英文摘要	Alpha-synuclein (alpha syn) is the main component of lewy bodies formed in midbrain dopaminergic neurons which is a pathological characteristic of parkinson's disease. it has been recently showed to induce endoplasmic reticulum (er) stress and impair er functions. however, the mechanism of how er responds to alpha syn toxicity is poorly understood. in the present study, we found that protein disulfide isomerase (pdi), a stress protein abundant in er, effectively inhibits alpha syn fibril formation in vitro. in pdi molecule with a structure of abb'xa'c, domain a' was found to be essential and sufficient for pdi to inhibit alpha syn fibril formation. pdi was further found to be more avid for binding with intermediate species formed during alpha syn fibril formation, and the binding was more intensive in the later lag phase. our results provide new insight into the role of pdi in protecting er from the deleterious effects of misfolded protein accumulation in many neurodegenerative diseases.
WOS关键词	ENDOPLASMIC-RETICULUM STRESS ; PARKINSONS-DISEASE ; MOLECULAR CHAPERONES ; BINDING SITE ; IN-VITRO ; MUTATION ; FAMILY ; GENE ; IDENTIFICATION ; PATHOGENESIS
WOS研究方向	Cell Biology
WOS类目	Cell Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000278681300008
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2413055
专题	中国科学院大学
通讯作者	Wang, Chih-chen
作者单位	1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Han,Wang, Lei,Wang, Chih-chen. Domain a' of protein disulfide isomerase plays key role in inhibiting alpha-synuclein fibril formation[J]. Cell stress & chaperones,2010,15(4):415-421.
APA	Cheng, Han,Wang, Lei,&Wang, Chih-chen.(2010).Domain a' of protein disulfide isomerase plays key role in inhibiting alpha-synuclein fibril formation.Cell stress & chaperones,15(4),415-421.
MLA	Cheng, Han,et al."Domain a' of protein disulfide isomerase plays key role in inhibiting alpha-synuclein fibril formation".Cell stress & chaperones 15.4(2010):415-421.

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