| Overexpression of osran2 in rice and arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress | |
| Zang, Aiping1; Xu, Xiaojie1; Neill, Steven2; Cai, Weiming1 | |
| 刊名 | Journal of experimental botany
 |
| 2010-03-01 | |
| 卷号 | 61期号:3页码:777-789 |
| 关键词 | Abiotic stress Arabidopsis Nuclear-cytoplasmic trafficking Osran2 Rice |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/erp341 |
| 通讯作者 | Cai, weiming(wmcai@sippe.ac.cn) |
| 英文摘要 | Nucleo-cytoplasmic partitioning of regulatory proteins is increasingly being recognized as a major control mechanism for the regulation of signalling in plants. ras-related nuclear protein (ran) gtpase is required for regulating transport of proteins and rna across the nuclear envelope and also has roles in mitotic spindle assembly and nuclear envelope (ne) assembly. however, thus far little is known of any ran functions in the signalling pathways in plants in response to changing environmental stimuli. the osran2 gene, which has high homology (77% at the amino acid level) with its human counterpart, was isolated here. subcellular localization results showed that osran2 is mainly localized in the nucleus, with some in the cytoplasm. transcription of osran2 was reduced by salt, osmotic, and exogenous abscisic acid (aba) treatments, as determined by real-time pcr. overexpression of osran2 in rice resulted in enhanced sensitivity to salinity, osmotic stress, and aba. seedlings of transgenic arabidopsis thaliana plants overexpressing osran2 were overly sensitive to salinity stress and exogenous aba treatment. furthermore, three aba- or stress-responsive genes, atnced3, atplc1, and atmyb2, encoding a key enzyme in aba synthesis, a phospholipase c homologue, and a putative transcriptional factor, respectively, were shown to have differentially induced expression under salinity and aba treatments in transgenic and wild-type arabidopsis plants. osran2 overexpression in tobacco epidermal leaf cells disturbed the nuclear import of a maize (zea mays l.) leaf colour transcription factor (lc). in addition, gene-silenced rice plants generated via rna interference (rnai) displayed pleiotropic developmental abnormalities and were male sterile. |
| WOS关键词 | GTP-BINDING PROTEIN ; ORYZA-SATIVA L ; GENE-EXPRESSION ; SIGNAL-TRANSDUCTION ; NUCLEAR-LOCALIZATION ; MITOTIC PROGRESS ; PHOSPHOLIPASE-C ; SALT TOLERANCE ; MALE-STERILITY ; IMPORTIN BETA |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000274345000015 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2408026 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Cai, Weiming |
| 作者单位 | 1.Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Univ W England, Ctr Res Plant Sci, Bristol BS16 1QY, Avon, England |
| 推荐引用方式 GB/T 7714 | Zang, Aiping,Xu, Xiaojie,Neill, Steven,et al. Overexpression of osran2 in rice and arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress[J]. Journal of experimental botany,2010,61(3):777-789. |
| APA | Zang, Aiping,Xu, Xiaojie,Neill, Steven,&Cai, Weiming.(2010).Overexpression of osran2 in rice and arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress.Journal of experimental botany,61(3),777-789. |
| MLA | Zang, Aiping,et al."Overexpression of osran2 in rice and arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress".Journal of experimental botany 61.3(2010):777-789. |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论