| Pcaf acetylates beta-catenin and improves its stability | |
| Ge, Xinjian; Jin, Qihuang; Zhang, Fang; Yan, Tingting; Zhai, Qiwei1 | |
| 刊名 | Molecular biology of the cell
 |
| 2009 | |
| 卷号 | 20期号:1页码:419-427 |
| ISSN号 | 1059-1524 |
| DOI | 10.1091/mbc.e08-08-0792 |
| 通讯作者 | Zhai, qiwei(qwzhai@sibs.ac.cn) |
| 英文摘要 | Beta-catenin plays an important role in development and tumorigenesis. however, the effect of a key acetyltransferase p300/cbp-associated factor (pcaf) on beta-catenin signaling is largely unknown. in this study, we found pcaf could increase the beta-catenin transcriptional activity, induce its nuclear translocation, and up-regulate its protein level by inhibiting its ubiquitination and improving its stability. further studies showed that pcaf directly binds to and acetylates beta-catenin. the key ubiquitination sites lys-19 and lys-49 of beta-catenin were shown as the critical residues for pcaf-induced acetylation and stabilization. knockdown of pcaf in colon cancer cells markedly reduced the protein level, transcriptional activity, and acetylation level of beta-catenin; promoted cell differentiation; inhibited cell migration; and repressed xenografted tumorigenesis and tumor growth in nude mice. all these data demonstrate that pcaf acetylates beta-catenin and regulates its stability, and they raise the prospect that therapies targeting pcaf may be of clinical use in beta-catenin-driven diseases, such as colon cancer. |
| WOS关键词 | GLYCOGEN-SYNTHASE KINASE-3 ; COLON-CANCER ; SIRT1 DEACETYLASE ; TRANSCRIPTIONAL COACTIVATOR ; MOLECULAR TARGET ; CELL-SURVIVAL ; DNA-DAMAGE ; IN-VITRO ; PATHWAY ; PROTEIN |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| 出版者 | AMER SOC CELL BIOLOGY |
| WOS记录号 | WOS:000262134800039 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2404731 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhai, Qiwei |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Nutr & Metab, Inst Nutr Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Xinjian,Jin, Qihuang,Zhang, Fang,et al. Pcaf acetylates beta-catenin and improves its stability[J]. Molecular biology of the cell,2009,20(1):419-427. |
| APA | Ge, Xinjian,Jin, Qihuang,Zhang, Fang,Yan, Tingting,&Zhai, Qiwei.(2009).Pcaf acetylates beta-catenin and improves its stability.Molecular biology of the cell,20(1),419-427. |
| MLA | Ge, Xinjian,et al."Pcaf acetylates beta-catenin and improves its stability".Molecular biology of the cell 20.1(2009):419-427. |
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