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	Identification of elevated transcripts in a trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization
	Su, Xiaoyun1,2; Chu, Xin1,2; Dong, Zhiyang1
刊名	World journal of microbiology & biotechnology
	2009-06-01
卷号	25期号:6页码:1075-1084
关键词	Trichoderma reesei Chimeric transcription activator Suppression subtractive hybridization Cre1 Acei Aceii
ISSN号	0959-3993
DOI	10.1007/s11274-009-9993-6
通讯作者	Dong, zhiyang(dongzy@sun.im.ac.cn)
英文摘要	To know how the two transcription repressors cre1 and acei affect their downstream genes, a new strategy was employed in which a plasmid encoding a chimeric transcription activator containing the dna binding domains from cre1 and acei and the effector domain from aceii was constructed and transformed into trichoderma reesei. nineteen elevated transcripts were identified in the transformant against its parent strain using suppression subtractive hybridization. all of them had the consensus binding motif for cre1 (5'-syggrg-3') and acei (5'-aggca-3'), among which seven had the most representative binding motif (5'-aggcaaa-3') for acei in their 1,000-bp promoters. cre1 could bind to the promoters of all the genes while acei could bind to the promoters of six out of the seven in vitro. the results provide a primary overview of a set of genes which may be associated to cre1 or acei. it is the first step towards an understanding of the regulatory roles of the two repressors in cellular pathways, which would be helpful in strain improvement in t. reesei.
WOS关键词	CARBON CATABOLITE REPRESSION ; ASPERGILLUS-NIDULANS ; GLUCOSE REPRESSION ; GENE-EXPRESSION ; BINDING-SITES ; CELLULASE ; CLONING ; GROWTH ; TRANSFORMATION ; RECOGNITION
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000266168800017
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2399114
专题	中国科学院大学
通讯作者	Dong, Zhiyang
作者单位	1.Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Su, Xiaoyun,Chu, Xin,Dong, Zhiyang. Identification of elevated transcripts in a trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization[J]. World journal of microbiology & biotechnology,2009,25(6):1075-1084.
APA	Su, Xiaoyun,Chu, Xin,&Dong, Zhiyang.(2009).Identification of elevated transcripts in a trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization.World journal of microbiology & biotechnology,25(6),1075-1084.
MLA	Su, Xiaoyun,et al."Identification of elevated transcripts in a trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization".World journal of microbiology & biotechnology 25.6(2009):1075-1084.

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