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	Nitric oxide modulates the influx of extracellular ca2+ and actin filament organization during cell wall construction in pinus bungeana pollen tubes
	Wang, Yuhua1,2,3; Chen, Tong1; Zhang, Chunyang1,2; Hao, Huaiqing1; Liu, Peng1,2; Zheng, Maozhong1,2; Baluska, Frantisek4; Samaj, Jozef4,5,6; Lin, Jinxing1
刊名	New phytologist
	2009
卷号	182期号:4页码:851-862
关键词	Actin filaments Calcium influx Cell wall Nitric oxide (no) Pollen tube
ISSN号	0028-646X
DOI	10.1111/j.1469-8137.2009.02820.x
通讯作者	Lin, jinxing(linjx@ibcas.ac.cn)
英文摘要	Nitric oxide (no) plays a key role in many physiological processes in plants, including pollen tube growth. here, effects of no on extracellular ca2+ flux and microfilaments during cell wall construction in pinus bungeana pollen tubes were investigated. extracellular ca2+ influx, the intracellular ca2+ gradient, patterns of actin organization, vesicle trafficking and cell wall deposition upon treatment with the no donor s-nitroso-n-acetylpenicillamine (snap), the no synthase (nos) inhibitor n.-nitro-l-arginine (l-nna) or the no scavenger 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (cptio) were analyzed. snap enhanced pollen tube growth in a dose-dependent manner, while l-nna and cptio inhibited no production and arrested pollen tube growth. noninvasive detection and microinjection of a ca2+ indicator revealed that snap promoted extracellular ca2+ influx and increased the steepness of the tip-focused ca2+ gradient, while cptio and l-nna had the opposite effect. fluorescence labeling indicated that snap, cptio and l-nna altered actin organization, which subsequently affected vesicle trafficking. finally, the configuration and/or distribution of cell wall components such as pectins and callose were significantly altered in response to l-nna. fourier transform infrared (ftir) microspectroscopy confirmed the changes in the chemical composition of walls. our results indicate that no affects the configuration and distribution of cell wall components in pollen tubes by altering extracellular ca2+ influx and f-actin organization.
WOS关键词	ADVENTITIOUS ROOT-FORMATION ; PROTEIN-KINASES ; CALCIUM INFLUX ; BREFELDIN-A ; TIP GROWTH ; PLANTS ; POLYMERIZATION ; TRAFFICKING ; INVOLVEMENT ; GERMINATION
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000265936500010
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2397853
专题	中国科学院大学
通讯作者	Lin, Jinxing
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynthesis & Mol Environm Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China 4.Univ Bonn, Inst Cellular & Mol Bot, Dept Plant Cell Biol, D-53115 Bonn, Germany 5.Slovak Acad Sci, Inst Plant Genet & Biotechnol, SK-95007 Nitra, Slovakia 6.Univ Olomouc, Fac Nat Sci, Olomouc 77146, Czech Republic
推荐引用方式 GB/T 7714	Wang, Yuhua,Chen, Tong,Zhang, Chunyang,et al. Nitric oxide modulates the influx of extracellular ca2+ and actin filament organization during cell wall construction in pinus bungeana pollen tubes[J]. New phytologist,2009,182(4):851-862.
APA	Wang, Yuhua.,Chen, Tong.,Zhang, Chunyang.,Hao, Huaiqing.,Liu, Peng.,...&Lin, Jinxing.(2009).Nitric oxide modulates the influx of extracellular ca2+ and actin filament organization during cell wall construction in pinus bungeana pollen tubes.New phytologist,182(4),851-862.
MLA	Wang, Yuhua,et al."Nitric oxide modulates the influx of extracellular ca2+ and actin filament organization during cell wall construction in pinus bungeana pollen tubes".New phytologist 182.4(2009):851-862.

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