| Chiral ligand-exchange ce assays for separation of amino acid enantiomers and determination of enzyme kinetic constant | |
| Qi, Li1,3; Qiao, Juan1,2; Yang, Gengliang3; Chen, Yi1 | |
| 刊名 | Electrophoresis
 |
| 2009-07-01 | |
| 卷号 | 30期号:13页码:2266-2272 |
| 关键词 | D-amino acid oxidase Enantioseparation Enzyme kinetics Ligand-exchange ce Zinc complex |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200800623 |
| 通讯作者 | Qi, li(qili@iccas.ac.cn) |
| 英文摘要 | This paper deals with studies on the use of zn(ii)-l-ornithine complex as a chiral selecting system for the enantioseparation and uv detection of amino acids (aas) by using the principle of ligand-exchange ce. successful enantioseparation of three pairs of label-free aromatic aas and four pairs of labeled aa enantiomers have been achieved with a buffer of 100.0 mm boric acid, 5.0 mm ammonium acetate, 3.0 mm znso(4) and 6.0 mm l-orn at ph 8.2. this new method was shown to be applicable to the quantitative analysis of d- and l-aromatic aas, with a linear range between 12.5 and 800.0 mu g/ml, and a correlation coefficient above 0.99. thus this assay, which is facile and relatively rapid, allows us to measure the enzyme catalytic activity in the incubation of d,l-aas with d-aa oxidase. using this new method, we can determine the enzyme kinetic constant, lending insight into potential enzyme mechanism. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS METHOD ; NITRO-D-ARGININE ; D-SERINE ; ZONE-ELECTROPHORESIS ; BETA-CYCLODEXTRIN ; BRAIN-AREAS ; D-ASPARTATE ; D-LEUCINE ; OXIDASE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000268626900003 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2394571 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Analyt Chem Life Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China 3.Hebei Univ, Coll Pharm, Baoding, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Li,Qiao, Juan,Yang, Gengliang,et al. Chiral ligand-exchange ce assays for separation of amino acid enantiomers and determination of enzyme kinetic constant[J]. Electrophoresis,2009,30(13):2266-2272. |
| APA | Qi, Li,Qiao, Juan,Yang, Gengliang,&Chen, Yi.(2009).Chiral ligand-exchange ce assays for separation of amino acid enantiomers and determination of enzyme kinetic constant.Electrophoresis,30(13),2266-2272. |
| MLA | Qi, Li,et al."Chiral ligand-exchange ce assays for separation of amino acid enantiomers and determination of enzyme kinetic constant".Electrophoresis 30.13(2009):2266-2272. |
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