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	Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth
	Ma, Qing-Hu1; Liu, Yun-Chao1,2
刊名	Plant cell reports
	2009-11-01
卷号	28期号:11页码:1759-1765
关键词	Isopentenyl transferase gene (ipt) Leaf senescence Root growth Transgenic tobacco
ISSN号	0721-7714
DOI	10.1007/s00299-009-0776-1
通讯作者	Ma, qing-hu(mqh@ibcas.ac.cn)
英文摘要	A cytokinin biosynthetic gene encoding isopentenyl transferase (ipt) was cloned with its native promoter from agrobacterium tumefaciens and introduced into tobacco plants. indolebutyric acid was applied in rooting medium and morphologically normal transgenic tobacco plants were regenerated. genetic analysis of self-fertilized progeny showed that a single copy of intact ipt gene had been integrated, and t(2) progeny had become homozygous for the transgene. stable inheritance of the intact ipt gene in t(2) progeny was verified by southern hybridization. northern blot hybridization revealed that the expression of this ipt gene was confined in leaves and stems but undetectable in roots of the transgenic plants. endogenous cytokinin levels in the leaves and stems of the transgenic tobaccos were two to threefold higher than that of control, but in roots, both the transgenic and control tobaccos had similar cytokinin levels. the elevated cytokinin levels in the transgenic tobacco leaves resulted in delayed leaf senescence in terms of chlorophyll content without affecting the net photosynthetic rate. the root growth and morphology of the plant were not affected in the transgenic tobacco.
WOS关键词	TRANSFORMED PLANT-CELLS ; TRANSGENIC TOBACCO ; CYTOKININ BIOSYNTHESIS ; PROMOTER ; ARABIDOPSIS ; SEQUENCE ; AUXIN ; DNA ; METABOLISM ; SHOOT
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000271905900015
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2394367
专题	中国科学院大学
通讯作者	Ma, Qing-Hu
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Ma, Qing-Hu,Liu, Yun-Chao. Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth[J]. Plant cell reports,2009,28(11):1759-1765.
APA	Ma, Qing-Hu,&Liu, Yun-Chao.(2009).Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth.Plant cell reports,28(11),1759-1765.
MLA	Ma, Qing-Hu,et al."Expression of isopentenyl transferase gene (ipt) in leaf and stem delayed leaf senescence without affecting root growth".Plant cell reports 28.11(2009):1759-1765.

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