QQ客服 官方微博 反馈留言

	Fluorinated silicon surfaces under mixed surfactants: resistance to nonspecific protein adsorption for biosensing
	Hu, Kejia1,3; Gao, Yunhua1; Zhou, Wenwen1,3; Lian, Jie1,3; Li, Fang1; Chen, Zhifeng2
刊名	Langmuir
	2009-11-03
卷号	25期号:21页码:12404-12407
ISSN号	0743-7463
DOI	10.1021/la901588x
通讯作者	Gao, yunhua(yhgao@mail.ipc.ac.cn)
英文摘要	This paper describes a simple method for preparing protein microarrays that is compatible with high throughput manufacturing, the microarrays were formed by maskless photolithography and pin spotting to study protein adsorption on a fluorinated/methoxy-poly(ethylene glycol) (peg) self-assembled monolayer (sam). the mixed nonionic surfactants, tween20 and peg200, were utilized to control nonspecific protein adsorption oil both of the sams. measurements using double-antibody sandwich quantum dots-linked immunosorbent assay (das-qdlisa) showed that the fluorinated sam could effectively minimize nonspecific adsorption in the presence of tween 20 and peg 200 (inhibitors of nonspecific protein adsorption (inspas)) while the pegylated surface was biofouling. additionally, pin spotting was used to fabricate high-throughput protein arrays oil the fluorinated sam. the results displayed that fluorinated sam could not only effectively immobilize protein by hydrophobic interactions, but could also resist the other nonspecific adsorption with the inspa. in this way, protein microarrays would be formed more conveniently and environmentally friendly. it is believed that simple, practical, and high-throughput protein immunosensing could be established with these mixed nonionic surfactants.
WOS关键词	SELF-ASSEMBLED MONOLAYERS ; POLY(ETHYLENE GLYCOL) ; CHAIN DENSITY ; ADHESION ; GOLD
WOS研究方向	Chemistry ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000271106600002
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2393892
专题	中国科学院大学
通讯作者	Gao, Yunhua
作者单位	1.Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China 2.Chinese Acad Inspect & Quarantine, Beijing 100123, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Kejia,Gao, Yunhua,Zhou, Wenwen,et al. Fluorinated silicon surfaces under mixed surfactants: resistance to nonspecific protein adsorption for biosensing[J]. Langmuir,2009,25(21):12404-12407.
APA	Hu, Kejia,Gao, Yunhua,Zhou, Wenwen,Lian, Jie,Li, Fang,&Chen, Zhifeng.(2009).Fluorinated silicon surfaces under mixed surfactants: resistance to nonspecific protein adsorption for biosensing.Langmuir,25(21),12404-12407.
MLA	Hu, Kejia,et al."Fluorinated silicon surfaces under mixed surfactants: resistance to nonspecific protein adsorption for biosensing".Langmuir 25.21(2009):12404-12407.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。