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	Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in china
	Yu, Y.1,2; Wang, J.1,2; Zhu, M. -L.1,2; Wei, Z. -M.1
刊名	Plant breeding
	2008-06-01
卷号	127期号:3页码:249-255
关键词	Triticum aestivum Embryogenic callus In vitro regeneration Silver nitrate Cupric sulfate
ISSN号	0179-9541
DOI	10.1111/j.1439-0523.2007.01461.x
通讯作者	Wei, z. -m.(zmwei@sibs.ac.cn)
英文摘要	We have developed an efficient procedure for plant regeneration of elite wheat cultivars using mature embryos. firstly, we established the optimal combination of basal media, inoculation method and pretreatment method using biostatistical methods. the results indicated that the combination of ms medium and longitudinally bisected mature embryos showed the highest culture efficiency, whereas the pretreatment method had no significant effects on callus induction or plant regeneration. a 70% primary callus induction rate was achieved on ms medium containing 2 mg/l 2,4-d for all tested cultivars. primary calli were then transferred onto the subculture medium to initiate embryogenic calli. supplementation of the subculture medium with the appropriate combination of phytohormones (2.0 mg/l 2,4-d, 0.5 mg/l ba and 0.1 mg/l naa) significantly enhanced embryogenic callus production. the addition of agno(3) (10 mg/l) in regeneration medium promoted plant regeneration, whereas cuso(4) stimulated root formation. the use of this protocol achieved successful plant regeneration in eight tested cultivars. the culture efficiency ranged from 15.3% to 36.8%, suggesting this regeneration system may be an effective alternative for wheat genetic transformation.
WOS关键词	TRITICUM-AESTIVUM L ; TOBACCO TISSUE-CULTURES ; BARLEY HORDEUM-VULGARE ; SOMATIC EMBRYOGENESIS ; ANTHER CULTURE ; ETHYLENE INHIBITOR ; SHOOT REGENERATION ; WINTER-WHEAT ; IN-VITRO ; INITIATION
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Plant Sciences
WOS类目	Agronomy ; Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000255287500006
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2388959
专题	中国科学院大学
通讯作者	Wei, Z. -M.
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Yu, Y.,Wang, J.,Zhu, M. -L.,et al. Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in china[J]. Plant breeding,2008,127(3):249-255.
APA	Yu, Y.,Wang, J.,Zhu, M. -L.,&Wei, Z. -M..(2008).Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in china.Plant breeding,127(3),249-255.
MLA	Yu, Y.,et al."Optimization of mature embryo-based high frequency callus induction and plant regeneration from elite wheat cultivars grown in china".Plant breeding 127.3(2008):249-255.

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