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	Microinjection as a tool of mechanical delivery
	Zhang, Yan1; Yu, Long-Chuan
刊名	Current opinion in biotechnology
	2008-10-01
卷号	19期号:5页码:506-510
ISSN号	0958-1669
DOI	10.1016/j.copbio.2008.07.005
通讯作者	Zhang, yan(yanzhang@pku.edu.cn)
英文摘要	Microinjection to single cells has been widely used in the studies of transduction-challenged cells, transgenic animal production, and in vitro fertilization to mechanically transfer dnas, rna interferences, sperms, proteins, peptides, and drugs. the advantages of microinjection include the precision of delivery dosage and timing, high efficiency of transduction as well as low cytotoxicity. however, manual microinjection is labor intensive and time consuming, which limits the application of this technique to large number of cells in a sample. new cell culture matrix ensuring all cells grow in a desired position and orientation is needed for application of high throughput automatic injection systems, which will significantly increase injection speed, cell survival, and success rates.
WOS关键词	EMBRYONIC STEM-CELLS ; INTRACYTOPLASMIC SPERM INJECTION ; XENOPUS-LAEVIS OOCYTES ; HUMAN PRIMARY NEURONS ; PRION PROTEIN ; ANTISENSE OLIGONUCLEOTIDES ; GENE-TRANSFER ; ELECTROPORATION ; EXPRESSION ; BAX
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology
语种	英语
出版者	CURRENT BIOLOGY LTD
WOS记录号	WOS:000260387900014
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2388644
专题	中国科学院大学
通讯作者	Zhang, Yan
作者单位	1.Peking Univ, Neurobiol Lab, Beijing 100871, Peoples R China 2.Peking Univ, State Key Lab Biomembrane & Membrane Biotechnol, Coll Life Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yan,Yu, Long-Chuan. Microinjection as a tool of mechanical delivery[J]. Current opinion in biotechnology,2008,19(5):506-510.
APA	Zhang, Yan,&Yu, Long-Chuan.(2008).Microinjection as a tool of mechanical delivery.Current opinion in biotechnology,19(5),506-510.
MLA	Zhang, Yan,et al."Microinjection as a tool of mechanical delivery".Current opinion in biotechnology 19.5(2008):506-510.

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